| Host: | Rabbit |
| Applications: | CM/ELISA/ICC/IF/IHC/IP/WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-PDE4A (N-Term) is suitable for use in Confocal Microscopy, ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Biotin |
| Isotype: | IgG |
| Formulation: | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Purification: | Affinity Purified |
| Concentration: | 0.70-1.0 µg/µl |
| Dilution Range: | WB: 1:750-1:1, 000 DB: 1:10, 000 ELISA: 1:10, 000 IP: 1:250 IHC: 1:250 ICC: 1:250 IF: 1:250 CFM: 1:250 |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | PDE4A |
| Gene ID: | 5141 |
| Uniprot ID: | PDE4A_HUMAN |
| Immunogen Region: | N-Term |
| Specificity: | Does not crossDoes not cross-react with other PDE family members. |
| Immunogen: | Synthetic cyclic peptide specific only for PDE4A5 protein. N-terminal sequence modified to achieve desired antigenicity and linker for covalent coupling to blue carrier protein. |
| Tissue Specificity | Isoform 1: Expressed in lymphoid cell subsets including CD8-positive T cells and T-helper 2 cells. Expressed in dendritic cells. Isoform 2: Highly expressed in liver, stomach, testis, thyroid and adrenal glands and at a lower extent in placenta, kidney, pancreas, ovary, uterus and skin. Expressed in myeloid cell subsets including dendritic cells, monocytes, macrophages, eosinophils and mast cells. Expressed in natural killer cells. Expressed in bronchial smooth muscle. Isoform 6: Expressed at high levels in the heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and peripheral blood lymphocytes. Isoform 7: Expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Found in specific neuronal subpopulations including cortical pyramidal neurons, horn neurons in the spinal cord and Purkinje cells in cerebellum (at protein level). |
| Post Translational Modifications | Isoform 1: Proteolytically cleaved by CASP3. Isoform 2: Phosphorylated at Ser-119 by PKA. |
| Function | Hydrolyzes the second messenger 3',5'-cyclic AMP (cAMP), which is a key regulator of many important physiological processes. Isoform 1: Efficiently hydrolyzes cAMP. Isoform 2: Efficiently hydrolyzes cAMP. Isoform 3: Efficiently hydrolyzes cAMP. The phosphodiesterase activity is not affected by calcium, calmodulin or cyclic GMP (cGMP) levels. Does not hydrolyze cGMP. Isoform 4: Efficiently hydrolyzes cAMP. Isoform 6: Efficiently hydrolyzes cAMP. Isoform 7: Efficiently hydrolyzes cAMP. |
| Protein Name | 3' -5'-Cyclic-Amp Phosphodiesterase 4a Dpde2 Pde46 Camp-Specific Phosphodiesterase 4a |
| Database Links | Reactome: R-HSA-180024 Reactome: R-HSA-418555 |
| Cellular Localisation | Isoform 1: Cytoplasm Perinuclear Region Isoform 2: Cytoplasm Cell Projection Ruffle Membrane Isoform 3: Cytoplasm Cytosol Isoform 4: Membrane Peripheral Membrane Protein Isoform 4 Has Propensity For Association With Membranes Isoform 6: Cytoplasm Isoform 7: Cytoplasm Membrane Predominantly Cytosolic |
| Alternative Antibody Names | Anti-3' -5'-Cyclic-Amp Phosphodiesterase 4a antibody Anti-Dpde2 antibody Anti-Pde46 antibody Anti-Camp-Specific Phosphodiesterase 4a antibody Anti-PDE4A antibody Anti-DPDE2 antibody |
Information sourced from Uniprot.org