Anti-OCT-3/4 antibody [C-10] (STJ16101853)

SKU:
STJ16101853-1

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Host: Mouse
Applications: IHC-F/IHC-P
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-OCT-3/4 is suitable for use in Immunohistochemistry and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: C-10
Conjugation: Unconjugated
Isotype: IgG2b
Formulation: Purified antibody in PBS with 0.2 % BSA and 15mM sodium azide
Purification: Purified
Dilution Range: IHC-F
IHC-P 1:50-1:100
Storage Instruction: Store at 2-8°C upon receipt.
Immunogen: Amino acids1-134 of Oct-3/4 of human origin
Background Transcription factors containing the POU homeo domain have been shown to be important regulators of tissue-specific gene expression in lymphoid and pituitary differentiation and in early mammalian development. POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. Oct-3 (also known as Oct-4) is a mammalian POU transcription factor expressed by early embryo cells and germ cells. Oct-3/4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo. A critical amount of Oct-3/4 is required to sustain stem-cell self renewal, and up or down regulation induce divergent developmental programs. Two isoforms of Oct-3, termed Oct-3A and Oct-3B, are generated by alternative splicing. The gene which encodes Oct-3/4 maps to human chromosome 6p21.3. Oct-3/4 (C-10) is recommended for detection of Oct-3A (Oct-4) and Oct-3B of mouse, rat and human origin by Western Blotting, immunoprecipitation, immunofluorescence, and paraffin immunohistochemistry. Pre-treatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0, or in 50 mM Tris buffer pH9.5, for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based detection is recommended. Control tissue Seminoma or embryonal carcinoma. Staining Nuclear

Information sourced from Uniprot.org

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