Western blot analysis of extracts of mouse heart, using NT5C3A antibody (STJ29730) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-NT5C3A antibody (37-336) (STJ29730)

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STJ29730
£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NT5C3A
Gene ID: 51251
Uniprot ID: 5NT3A_HUMAN
Immunogen Region: 37-336
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 37-336 of human NT5C3A (NP_001002010.1).
Immunogen Sequence: TLKRKTGRKTKIIEMMPEFQ KSSVRIKNPTRVEEIICGLI KGGAAKLQIITDFDMTLSRF SYKGKRCPTCHNIIDNCKLV TDECRKKLLQLKEKYYAIEV DPVLTVEEKYPYMVEWYTKS HGLLVQQALPKAKLKEIVAE SDVMLKEGYENFFDKLQQHS IPVFIFSAGIGDVLEEVIRQ AGVYHPNVKVVSNFMDFDET GVLKGFKGELIHVFNKHDGA LRNTEYFNQLKDNSNIILL
Tissue Specificity Isoforms 1, 3 and 4 are expressed in reticulocytes. Isoform 4 is hardly detectable in bone marrow and fetal liver.
Function Nucleotidase which shows specific activity towards cytidine monophosphate (CMP) and 7-methylguanosine monophosphate (m(7)GMP). CMP seems to be the preferred substrate.
Protein Name Cytosolic 5'-Nucleotidase 3a
7-Methylguanosine Phosphate-Specific 5'-Nucleotidase
7-Methylguanosine Nucleotidase
Cytosolic 5'-Nucleotidase 3
Cytosolic 5'-Nucleotidase Iii
Cn-Iii
Pyrimidine 5'-Nucleotidase 1
P5'N-1
P5n-1
Pn-I
Uridine 5'-Monophosphate Hydrolase 1
P36
Database Links Reactome: R-HSA-73621 Q9H0P0-4
Cellular Localisation Cytoplasm
Isoform 2: Endoplasmic Reticulum
Alternative Antibody Names Anti-Cytosolic 5'-Nucleotidase 3a antibody
Anti-7-Methylguanosine Phosphate-Specific 5'-Nucleotidase antibody
Anti-7-Methylguanosine Nucleotidase antibody
Anti-Cytosolic 5'-Nucleotidase 3 antibody
Anti-Cytosolic 5'-Nucleotidase Iii antibody
Anti-Cn-Iii antibody
Anti-Pyrimidine 5'-Nucleotidase 1 antibody
Anti-P5'N-1 antibody
Anti-P5n-1 antibody
Anti-Pn-I antibody
Anti-Uridine 5'-Monophosphate Hydrolase 1 antibody
Anti-P36 antibody
Anti-NT5C3A antibody
Anti-NT5C3 antibody
Anti-P5N1 antibody
Anti-UMPH1 antibody
Anti-HSPC233 antibody

Information sourced from Uniprot.org

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