• Western blot analysis of various lysates using NSUN4 Rabbit polyclonal antibody (STJ117182) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-NSUN4 antibody (26-290) (STJ117182)

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STJ117182

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NSUN4 (26-290) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NSUN4
Gene ID: 387338
Uniprot ID: NSUN4_HUMAN
Immunogen Region: 26-290
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 26-290 of human NSUN4 (NP_950245.2).
Immunogen Sequence: RYKKKWAATEPKFPAVRLAL QNFDMTYSVQFGDLWPSIRV SLLSEQKYGALVNNFAAWDH VSAKLEQLSAKDFVNEAISH WELQSEGGQSAAPSPASWAC SPNLRCFTFDRGDISRFPPA RPGSLGVMEYYLMDAASLLP VLALGLQPGDIVLDLCAAPG GKTLALLQTGCCRNLAANDL SPSRIARLQKILHSYVPEEI RDGNQVRVTSWDGRKWGELE GDTYDRVLVDVPCTTDRHS
Function Involved in mitochondrial ribosome assembly. 5-methylcytosine rRNA methyltransferase that probably is involved in mitochondrial ribosome small subunit (SSU) maturation by methylation of mitochondrial 12S rRNA.the function is independent of MTERFD2/MTERF4 and assembled mitochondrial ribosome large subunit (LSU). Targeted to LSU by MTERFD2/MTERF4 and probably is involved in a final step in ribosome biogenesis to ensure that SSU and LSU are assembled. In vitro can methylate 16S rRNA of the LSU.the methylation is enhanced by MTERFD/MTERF4.
Protein Name 5-Methylcytosine Rrna Methyltransferase Nsun4
5-Methylcytosine Trna Methyltransferase Nsun4
Nol1/Nop2/Sun Domain Family Member 4
Database Links Reactome: R-HSA-6793080
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-5-Methylcytosine Rrna Methyltransferase Nsun4 antibody
Anti-5-Methylcytosine Trna Methyltransferase Nsun4 antibody
Anti-Nol1/Nop2/Sun Domain Family Member 4 antibody
Anti-NSUN4 antibody

Information sourced from Uniprot.org

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