Anti-NR2C1 antibody (STJ190193)

SKU:
STJ190193
Current Stock:
Host: Rabbit
Applications: WB, ELISA
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal primary antibody against NR2C1.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Purification: NR2C1 antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG
Dilution Range: WB 1:500-2000
ELISA 1:5000-20000
Specificity: NR2C1 polyclonal antibody detects endogenous levels of protein.
Uniprot ID: NR2C1_HUMAN
Gene ID: 7181
Gene Symbol: NR2C1
Immunogen: Synthesized peptide derived from human NR2C1 protein.
Immunogen Region: 40-120aa
Post Translational Modifications Sumoylation requires both PIAS1 and UBE2I. Sumoylation appears to dissociate NR2C1 from the PML nuclear bodies. Enhances the interaction with NRIP1 but inhibits interaction with KAT2B. In proliferating cells, stimulation by all-trans retinoic acid, activation of MAPK1-mediated phosphorylation and recruitment to PML bodies with subsequent sumoylation, suppresses OCT4 expression. Phosphorylated on several serine and threonine residues. Phosphorylation on Thr-222, stimulated by all-trans retinoic acid (atRA) mediates PML location and sumoylation in proliferating cells which then modulates its association with effector molecules, KAT2B and NRIP1. Phosphorylation on Ser-581 by PKC is important for protein stability and function as activator of RARB.
Function Orphan nuclear receptor. Binds the IR7 element in the promoter of its own gene in an autoregulatory negative feedback mechanism. Primarily repressor of a broad range of genes. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences. Together with NR2C2, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription. Also activator of OCT4 gene expression. May be involved in stem cell proliferation and differentiation. Mediator of retinoic acid-regulated preadipocyte proliferation.
Database Links Reactome: R-HSA-383280
Cellular Localisation Nucleus
Pml Body
Recruited By Hdac3
After All-Trans Retinoic Acid Stimulated Mapk1-Mediated Thr-223 Phosphorylation
To Pml Bodies For Subsequent Sumoylation
Protein Name Nuclear Receptor Subfamily 2 Group C Member 1
Orphan Nuclear Receptor Tr2
Testicular Receptor 2
Alternative Names Anti-Nuclear Receptor Subfamily 2 Group C Member 1 antibody
Anti-Orphan Nuclear Receptor Tr2 antibody
Anti-Testicular Receptor 2 antibody
Anti-NR2C1 antibody
Anti-TR2 antibody

Information sourced from Uniprot.org

6 months standard