• Western blot analysis of extracts of various cell lines, using NFYA antibody (STJ111124) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-NFYA antibody (1-318) (STJ111124)

SKU:
STJ111124

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NFYA (1-318) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NFYA
Gene ID: 4800
Uniprot ID: NFYA_HUMAN
Immunogen Region: 1-318
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-318 of human NFYA (NP_068351.1).
Immunogen Sequence: MEQYTANSNSSTEQIVVQAG QIQQQVQGQPLMVQVSGGQL ITSTGQPIMVQAVPGGQGQT IMQVPVSGTQGLQQIQLVPP GQIQIQGGQAVQVQGQQGQT QQIIIQQPQTAVTAGQTQTQ QQIAVQGQQVAQTAEGQTIV YQPVNADGTILQQVTVPVSG MITIPAASLAGAQIVQTGAN TNTTSSGQGTVTVTLPVAGN VVNSGGMVMMVPGAGSVPAI QRIPLPGAEMLEEEPLYVN
Function Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors. NF-YA positively regulates the transcription of the core clock component BMAL1.
Protein Name Nuclear Transcription Factor Y Subunit Alpha
Caat Box Dna-Binding Protein Subunit A
Nuclear Transcription Factor Y Subunit A
Nf-Ya
Database Links Reactome: R-HSA-1989781
Reactome: R-HSA-2426168
Reactome: R-HSA-380994
Reactome: R-HSA-381183
Reactome: R-HSA-9614657
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Nuclear Transcription Factor Y Subunit Alpha antibody
Anti-Caat Box Dna-Binding Protein Subunit A antibody
Anti-Nuclear Transcription Factor Y Subunit A antibody
Anti-Nf-Ya antibody
Anti-NFYA antibody

Information sourced from Uniprot.org

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