| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | NFATC2 |
| Gene ID: | 4773 |
| Uniprot ID: | NFAC2_HUMAN |
| Immunogen Region: | 1-300 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human NFATC2 (NP_775114.1). |
| Immunogen Sequence: | MNAPERQPQPDGGDAPGHEP GGSPQDELDFSILFDYEYLN PNEEEPNAHKVASPPSGPAY PDDVLDYGLKPYSPLASLSG EPPGRFGEPDRVGPQKFLSA AKPAGASGLSPRIEITPSHE LIQAVGPLRMRDAGLLVEQP PLAGVAASPRFTLPVPGFEG YREPLCLSPASSGSSASFIS DTFSPYTSPCVSPNNGGPDD LCPQFQNIPAHYSPRTSPIM SPRTSLAEDSCLGRHSPVP |
| Tissue Specificity | Expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas. Isoform 1 is highly expressed in the small intestine, heart, testis, prostate, thymus, placenta and thyroid. Isoform 3 is highly expressed in stomach, uterus, placenta, trachea and thyroid. |
| Post Translational Modifications | In resting cells, phosphorylated by NFATC-kinase on at least 18 sites in the 99-363 region. Upon cell stimulation, all these sites except Ser-243 are dephosphorylated by calcineurin. Dephosphorylation induces a conformational change that simultaneously exposes an NLS and masks an NES, which results in nuclear localization. Simultaneously, Ser-53 or Ser-56 is phosphorylated.which is required for full transcriptional activity. Ubiquitinated in endothelial cells by RNF213 downstream of the non-canonical Wnt signaling pathway, leading to its degradation by the proteasome. |
| Function | Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. Promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway. Is involved in the negative regulation of chondrogenesis. Recruited by AKAP5 to ORAI1 pore-forming subunit of CRAC channels in Ca(2+) signaling microdomains where store-operated Ca(2+) influx is coupled to calmodulin and calcineurin signaling and activation of NFAT-dependent transcriptional responses. |
| Protein Name | Nuclear Factor Of Activated T-Cells - Cytoplasmic 2 Nf-Atc2 Nfatc2 Nfat Pre-Existing Subunit Nf-Atp T-Cell Transcription Factor Nfat1 |
| Database Links | Reactome: R-HSA-2025928 Reactome: R-HSA-2871809 Reactome: R-HSA-5607763 Reactome: R-HSA-8877330 |
| Cellular Localisation | Cytoplasm Nucleus Cytoplasmic For The Phosphorylated Form And Nuclear After Activation That Is Controlled By Calcineurin-Mediated Dephosphorylation Rapid Nuclear Exit Of Nfatc Is Thought To Be One Mechanism By Which Cells Distinguish Between Sustained And Transient Calcium Signals The Subcellular Localization Of Nfatc Plays A Key Role In The Regulation Of Gene Transcription |
| Alternative Antibody Names | Anti-Nuclear Factor Of Activated T-Cells - Cytoplasmic 2 antibody Anti-Nf-Atc2 antibody Anti-Nfatc2 antibody Anti-Nfat Pre-Existing Subunit antibody Anti-Nf-Atp antibody Anti-T-Cell Transcription Factor Nfat1 antibody Anti-NFATC2 antibody Anti-NFAT1 antibody Anti-NFATP antibody |
Information sourced from Uniprot.org