• Immunofluorescence analysis of paraffin-embedded Rat heart tissue using Myosin heavy chain Rabbit polyclonal antibody (STJ11105659) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunohistochemistry analysis of Myosin heavy chain in paraffin-embedded Mouse skeletal muscle tissue using Myosin heavy chain Rabbit polyclonal antibody (STJ11105659) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-Myosin heavy chain antibody (1730-1830) (STJ11105659)

SKU:
STJ11105659

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: IHC/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Myosin heavy chain (1730-1830) is suitable for use in Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: IHC-P 1:50-1:200IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 1730-1830
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1730-1830 of human Myosin heavy chain (NP_002462.2).
Immunogen Sequence: KKMESDLTQLQSEVEEAVQE CRNAEEKAKKAITDAAMMAE ELKKEQDTSAHLERMKKNME QTIKDLQHRLDEAEQIALKG GKKQLQKLEARVRELEGELE A
Background Cardiac muscle myosin is a hexamer consisting of two heavy chain subunits, two light chain subunits, and two regulatory subunits. This gene encodes the alpha heavy chain subunit of cardiac myosin. The gene is located approximately 4kb downstream of the gene encoding the beta heavy chain subunit of cardiac myosin. Mutations in this gene cause familial hypertrophic cardiomyopathy and atrial septal defect 3.

Information sourced from Uniprot.org

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