• Western blot analysis of extracts of Jurkat cells, using MYLK2 antibody (STJ116507) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-MYLK2 antibody (1-90) (STJ116507)

SKU:
STJ116507

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MYLK2 (1-90) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MYLK2
Gene ID: 85366
Uniprot ID: MYLK2_HUMAN
Immunogen Region: 1-90
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human MYLK2 (NP_149109.1).
Immunogen Sequence: MATENGAVELGIQNPSTDKA PKGPTGERPLAAGKDPGPPD PKKAPDPPTLKKDAKAPASE KGDGTLAQPSTSSQGPKGEG DRGGGPAEGS
Tissue Specificity Heart and skeletal muscles. Increased expression in the apical tissue compared to the interventricular septal tissue.
Function Implicated in the level of global muscle contraction and cardiac function. Phosphorylates a specific serine in the N-terminus of a myosin light chain.
Protein Name Myosin Light Chain Kinase 2 - Skeletal/Cardiac Muscle
Mlck2
Cellular Localisation Cytoplasm
Colocalizes With Phosphorylated Myosin Light Chain (Rlcp) At Filaments Of The Myofibrils
Alternative Antibody Names Anti-Myosin Light Chain Kinase 2 - Skeletal/Cardiac Muscle antibody
Anti-Mlck2 antibody
Anti-MYLK2 antibody

Information sourced from Uniprot.org

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