| Host: | Rabbit |
| Applications: | WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Major histocompatibility complex class I-related gene protein is suitable for use in Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MR1 |
| Gene ID: | 3140 |
| Uniprot ID: | HMR1_HUMAN |
| Specificity: | This antibody detects endogenous levels of MR1 at Human, Mouse, Rat |
| Immunogen: | Synthesized peptide derived from human MR1 |
| Post Translational Modifications | N-glycosylated. |
| Function | Antigen-presenting molecule specialized in displaying microbial pyrimidine-based metabolites to alpha-beta T cell receptors (TCR) on innate-type mucosal-associated invariant T (MAIT) cells. In complex with B2M preferentially presents riboflavin-derived metabolites to semi-invariant TRAV1-2 TCRs on MAIT cells, guiding immune surveillance of the microbial metabolome at mucosal epithelial barriers. Signature pyrimidine-based microbial antigens are generated via non-enzymatic condensation of metabolite intermediates of the riboflavin pathway with by-products arising from other metabolic pathways such as glycolysis. Typical potent antigenic metabolites are 5-(2-oxoethylideneamino)-6-D-ribitylaminouracil (5-OE-RU) and 5-(2-oxopropylideneamino)-6-D-ribitylaminouracil (5-OP-RU), products of condensation of 5-amino-6-D-ribityaminouracil (5-A-RU) with glyoxal or methylglyoxal by-products, respectively. May present microbial antigens to various TRAV1-2-negative MAIT cell subsets, providing for unique recognition of diverse microbes, including pathogens that do not synthesize riboflavin. Upon antigen recognition, elicits rapid innate-type MAIT cell activation to eliminate pathogenic microbes by directly killing infected cells. During T cell development, drives thymic selection and post-thymic terminal differentiation of MAIT cells in a process dependent on commensal microflora. Acts as an immune sensor of cancer cell metabolome. May present a tumor-specific or -associated metabolite essential for cancer cell survival to a pan-cancer TCR consisting of TRAV38.2-DV8*TRAJ31 alpha chain paired with a TRBV25.1*TRBJ2.3 beta chain on a non-MAIT CD8-positive T cell clone (MC.7.G5), triggering T cell-mediated killing of a wide range of cancer cell types. |
| Protein Name | Major Histocompatibility Complex Class I-Related Gene Protein Mhc Class I-Related Gene Protein Class I Histocompatibility Antigen-Like Protein |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein Endoplasmic Reticulum Membrane Golgi Apparatus Membrane Early Endosome Membrane Late Endosome Membrane In The Absence Of Antigen Remains Within The Endoplasmic Reticulum Where It Acts As A Metabolite Sensor Antigen Binding Triggers Trafficking Of The Ternary Complex To The Plasma Membrane After Presentation Most Of These Complexes Are Rapidly Internalized And Degraded Via Endocytosis A Small Subset Recycles Via Endosomes Back To The Plasma Membrane And May Thus Acquire And Present New Antigens That Do Not Efficiently Reach The Endoplasmic Reticulum Isoform 1: Cell Membrane Single-Pass Membrane Protein Isoform 3: Cell Membrane The Larger Proportion Remains In The Er In An Immature State The Subset That Reach Cell Surface Does It Through A B2m-Independent Pathway Isoform 4: Secreted |
| Alternative Antibody Names | Anti-Major Histocompatibility Complex Class I-Related Gene Protein antibody Anti-Mhc Class I-Related Gene Protein antibody Anti-Class I Histocompatibility Antigen-Like Protein antibody Anti-MR1 antibody |
Information sourced from Uniprot.org