• Western blot analysis of extracts of various cell lines, using MEKK3 Rabbit mAb (STJ11101930) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s.

Anti-MEKK3-antibody [ARC2074] (STJ11101930)

SKU:
STJ11101930

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Host: Rabbit
Applications: WB
Reactivity: Human, Mouse
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit monoclonal antibody anti-MEKK3 is suitable for use in Western Blot.
Clonality: Monoclonal
Clone ID: ARC2074
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Gene Symbol: MAP3K3
Gene ID: 4215
Uniprot ID: M3K3_HUMAN
Immunogen: A synthesized peptide derived from human MEKK3
Post Translational Modifications Phosphorylation at Ser-166 and Ser-337 by SGK1 inhibits its activity.
Function Component of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators.
Protein Name Mitogen-Activated Protein Kinase Kinase Kinase 3
Mapk/Erk Kinase Kinase 3
Mek Kinase 3
Mekk 3
Database Links Reactome: R-HSA-9020702
Alternative Antibody Names Anti-Mitogen-Activated Protein Kinase Kinase Kinase 3 antibody
Anti-Mapk/Erk Kinase Kinase 3 antibody
Anti-Mek Kinase 3 antibody
Anti-Mekk 3 antibody
Anti-MAP3K3 antibody
Anti-MAPKKK3 antibody
Anti-MEKK3 antibody

Information sourced from Uniprot.org

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