| Host: | Mouse |
| Applications: | IHC-F/IHC-P/WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-MDM2 is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality : | Monoclonal |
| Clone ID : | SMP14 |
| Conjugation: | Unconjugated |
| Isotype: | IgG1 |
| Formulation: | Affinity purified antibody in aqueous buffered solution containing ≤0.09% sodium azide |
| Purification: | Purified |
| Dilution Range: | IHC-F IHC-P 1:50-1:100 WB |
| Storage Instruction: | Store at 2-8°C upon receipt. |
| Gene Symbol: | MDM2 |
| Gene ID: | 4193 |
| Uniprot ID: | MDM2_HUMAN |
| Immunogen: | Human MDM2 aa. 154-167 |
| Tissue Specificity | Ubiquitous. Isoform Mdm2-A, isoform Mdm2-B, isoform Mdm2-C, isoform Mdm2-D, isoform Mdm2-E, isoform Mdm2-F and isoform Mdm2-G are observed in a range of cancers but absent in normal tissues. |
| Post Translational Modifications | Phosphorylation on Ser-166 by SGK1 activates ubiquitination of p53/TP53. Phosphorylated at multiple sites near the RING domain by ATM upon DNA damage.this prevents oligomerization and E3 ligase processivity and impedes constitutive p53/TP53 degradation. Autoubiquitination leads to proteasomal degradation.resulting in p53/TP53 activation it may be regulated by SFN. Also ubiquitinated by TRIM13. Deubiquitinated by USP2 leads to its accumulation and increases deubiquitination and degradation of p53/TP53. Deubiquitinated by USP7 leading to its stabilization. |
| Function | E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also a component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. Mediates ubiquitination and subsequent proteasome degradation of DYRK2 in nucleus. Ubiquitinates IGF1R and SNAI1 and promotes them to proteasomal degradation. Ubiquitinates DCX, leading to DCX degradation and reduction of the dendritic spine density of olfactory bulb granule cells. Ubiquitinates DLG4, leading to proteasomal degradation of DLG4 which is required for AMPA receptor endocytosis. Negatively regulates NDUFS1, leading to decreased mitochondrial respiration, marked oxidative stress, and commitment to the mitochondrial pathway of apoptosis. Binds NDUFS1 leading to its cytosolic retention rather than mitochondrial localization resulting in decreased supercomplex assembly (interactions between complex I and complex III), decreased complex I activity, ROS production, and apoptosis. |
| Protein Name | E3 Ubiquitin-Protein Ligase Mdm2 Double Minute 2 Protein Hdm2 Oncoprotein Mdm2 Ring-Type E3 Ubiquitin Transferase Mdm2 P53-Binding Protein Mdm2 |
| Database Links | Reactome: R-HSA-198323 Reactome: R-HSA-2559580 Reactome: R-HSA-2559585 Reactome: R-HSA-3232118 Reactome: R-HSA-3232142 Reactome: R-HSA-399719 Reactome: R-HSA-5674400 Reactome: R-HSA-5689880 Reactome: R-HSA-6804756 Reactome: R-HSA-6804757 Reactome: R-HSA-6804760 Reactome: R-HSA-69541 Reactome: R-HSA-8941858 Reactome: R-HSA-9725370 Reactome: R-HSA-9768919 |
| Cellular Localisation | Nucleus Nucleoplasm Cytoplasm Nucleolus Expressed Predominantly In The Nucleoplasm Interaction With Arf(P14) Results In The Localization Of Both Proteins To The Nucleolus The Nucleolar Localization Signals In Both Arf(P14) And Mdm2 May Be Necessary To Allow Efficient Nucleolar Localization Of Both Proteins Colocalizes With Rassf1 Isoform A In The Nucleus |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Mdm2 antibody Anti-Double Minute 2 Protein antibody Anti-Hdm2 antibody Anti-Oncoprotein Mdm2 antibody Anti-Ring-Type E3 Ubiquitin Transferase Mdm2 antibody Anti-P53-Binding Protein Mdm2 antibody Anti-MDM2 antibody |
Information sourced from Uniprot.org