| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MDFI |
| Gene ID: | 4188 |
| Uniprot ID: | MDFI_HUMAN |
| Immunogen Region: | 1-160 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human MDFI (NP_005577.1). |
| Immunogen Sequence: | MYQVSGQRPSGCDAPYGAPS AAPGPAQTLSLLPGLEVVTG STHPAEAAPEEGSLEEAATP MPQGNGPGIPQGLDSTDLDV PTEAVTCQPQGNPLGCTPLL PNDSGHPSELGGTRRAGNGA LGGPKAHRKLQTHPSLASQG SKKSKSSSKSTTSQIPLQAQ |
| Function | Inhibits the transactivation activity of the Myod family of myogenic factors and represses myogenesis. Acts by associating with Myod family members and retaining them in the cytoplasm by masking their nuclear localization signals. Can also interfere with the DNA-binding activity of Myod family members. Plays an important role in trophoblast and chondrogenic differentiation. Regulates the transcriptional activity of TCF7L1/TCF3 by interacting directly with TCF7L1/TCF3 and preventing it from binding DNA. Binds to the axin complex, resulting in an increase in the level of free beta-catenin. Affects axin regulation of the WNT and JNK signaling pathways. |
| Protein Name | Myod Family InhibitorMyogenic Repressor I-Mf |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-Myod Family Inhibitor antibodyAnti-Myogenic Repressor I-Mf antibodyAnti-MDFI antibody |
Information sourced from Uniprot.org