Anti-MAPK9 antibody (370-420) {FITC} (STJ501719)

SKU:
STJ501719-100

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Host: Chicken
Applications: ELISA/IHC/IP/WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Chicken polyclonal antibody anti-MAPK9 (370-420) is suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation and Western Blot research applications.
Clonality: Polyclonal
Conjugation: FITC
Isotype: IgY
Purification: Affinity Purified
Concentration: 0.5 µg/µl
Dilution Range: WB: 1:500
ELISA: 1:10, 000
IP: 1:150
IHC: 1:50-1:100
Storage Instruction: Store at-20°C for long term storage. Avoid freeze-thaw cycles.
Gene Symbol: MAPK9
Gene ID: 5601
Uniprot ID: MK09_HUMAN
Immunogen Region: 370-420
Immunogen: Synthetic peptide taken within amino acid region 370-420 on human Mitogen-activated protein kinase 9.
Post Translational Modifications Dually phosphorylated on Thr-183 and Tyr-185 by MAP2K7 and MAP2K4, which activates the enzyme. Autophosphorylated in vitro.
Function Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK9/JNK2. In turn, MAPK9/JNK2 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. In response to oxidative or ribotoxic stresses, inhibits rRNA synthesis by phosphorylating and inactivating the RNA polymerase 1-specific transcription initiation factor RRN3. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including TP53 and YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Upon T-cell receptor (TCR) stimulation, is activated by CARMA1, BCL10, MAP2K7 and MAP3K7/TAK1 to regulate JUN protein levels. Plays an important role in the osmotic stress-induced epithelial tight-junctions disruption. When activated, promotes beta-catenin/CTNNB1 degradation and inhibits the canonical Wnt signaling pathway. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-BMAL1 heterodimer and plays a role in the regulation of the circadian clock. Phosphorylates POU5F1, which results in the inhibition of POU5F1's transcriptional activity and enhances its proteasomal degradation. MAPK9 isoforms display different binding patterns: alpha-1 and alpha-2 preferentially bind to JUN, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it.
Protein Name Mitogen-Activated Protein Kinase 9
Map Kinase 9
Mapk 9
Jnk-55
Stress-Activated Protein Kinase 1a
Sapk1a
Stress-Activated Protein Kinase Jnk2
C-Jun N-Terminal Kinase 2
Database Links Reactome: R-HSA-2559580
Reactome: R-HSA-2871796
Reactome: R-HSA-450321
Reactome: R-HSA-450341
Cellular Localisation Cytoplasm
Nucleus
Colocalizes With Pou5f1 In The Nucleus
Alternative Antibody Names Anti-Mitogen-Activated Protein Kinase 9 antibody
Anti-Map Kinase 9 antibody
Anti-Mapk 9 antibody
Anti-Jnk-55 antibody
Anti-Stress-Activated Protein Kinase 1a antibody
Anti-Sapk1a antibody
Anti-Stress-Activated Protein Kinase Jnk2 antibody
Anti-C-Jun N-Terminal Kinase 2 antibody
Anti-MAPK9 antibody
Anti-JNK2 antibody
Anti-PRKM9 antibody
Anti-SAPK1A antibody

Information sourced from Uniprot.org

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