• Immunohistochemistry analysis of MAP4 in paraffin-embedded mouse kidney using MAP4 Rabbit polyclonal antibody (STJ116286) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from U-87MG cells, using MAP4 Rabbit polyclonal antibody (STJ116286) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MAP4 antibody (942-1152) (STJ116286)

SKU:
STJ116286

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MAP4 (942-1152) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAP4
Gene ID: 4134
Uniprot ID: MAP4_HUMAN
Immunogen Region: 942-1152
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 942-1152 of human MAP4 (NP_002366.2).
Immunogen Sequence: TENIKHQPGGGRAKVEKKTE AAATTRKPESNAVTKTAGPI ASAQKQPAGKVQIVSKKVSY SHIQSKCGSKDNIKHVPGGG NVQIQNKKVDISKVSSKCGS KANIKHKPGGGDVKIESQKL NFKEKAQAKVGSLDNVGHLP AGGAVKTEGGGSEAPLCPGP PAGEEPAISEAAPEAGAPTS ASGLNGHPTLSGGGDQREAQ TLDSQIQETSI
Post Translational Modifications Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly. Phosphorylation on Ser-787 negatively regulates MAP4 activity to promote microtubule assembly. Isoform 3 is phosphorylated on Ser-337 and Ser-338.
Function Non-neuronal microtubule-associated protein. Promotes microtubule assembly.
Protein Name Microtubule-Associated Protein 4
Map-4
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Recruitment To Microtubule Is Inhibited By Microtubules Polyglutamylation
Alternative Antibody Names Anti-Microtubule-Associated Protein 4 antibody
Anti-Map-4 antibody
Anti-MAP4 antibody

Information sourced from Uniprot.org

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