• Immunohistochemistry of paraffin-embedded rat brain using Map2 antibody (STJ114887) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded mouse brain using Map2 antibody (STJ114887) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using Map2 antibody (STJ114887) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 5s.

Anti-Map2 antibody (STJ114887)

SKU:
STJ114887
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Host: Rabbit
Applications: WB, IHC
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal primary antibody against MAP2.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
IHC: 1:50-1:200
Uniprot ID: MTAP2_HUMAN
Gene ID: 4133
Gene Symbol: MAP2
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 260-559 of human Map2 (NP_001034627.1).
Background This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described.
Post Translational Modifications Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly. Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. The interaction with KNDC1 enhances MAP2 threonine phosphorylation.
Function The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Projection
Dendrite
Protein Name Microtubule-Associated Protein 2
Map-2
Alternative Names Anti-Microtubule-Associated Protein 2 antibody
Anti-Map-2 antibody
Anti-MAP2 antibody

Information sourced from uniprot.org

6 months standard