| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-LIME1 (1-295) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
LIME1 |
| Gene ID: |
54923 |
| Uniprot ID: |
LIME1_HUMAN |
| Immunogen Region: |
1-295 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-295 of human LIME1 (NP_060276.2). |
| Immunogen Sequence: |
MGLPVSWAPPALWVLGCCAL LLSLWALCTACRRPEDAVAP RKRARRQRARLQGSATAAEA SLLRRTHLCSLSKSDTRLHE LHRGPRSSRALRPASMDLLR PHWLEVSRDITGPQAAPSAF PHQELPRALPAAAATAGCAG LEATYSNVGLAALPGVSLAA SPVVAEYARVQKRKGTHRSP QEPQQGKTEVTPAAQVDVLY SRVCKPKRRDPGPTTDPLDP KGQGAILALAGDLAYQTLP |
| Tissue Specificity | Expressed in peripheral blood lymphocytes, lymphoid tissues, and liver. Present in T-cells and plasma cells, and in various hematopoietic cell lines (at protein level). |
| Post Translational Modifications | Palmitoylation of Cys-28 and Cys-31 is required for raft targeting. Phosphorylated on tyrosines upon TCR activation and/or CD4 coreceptor stimulation, or upon BCR stimulation.which leads to the recruitment of SH2-containing proteins. |
| Function | Involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and TCR (T-cell antigen receptor)-mediated T-cell signaling in T-cells. In absence of TCR signaling, may be involved in CD4-mediated inhibition of T-cell activation. Couples activation of these receptors and their associated kinases with distal intracellular events such as calcium mobilization or MAPK activation through the recruitment of PLCG2, GRB2, GRAP2, and other signaling molecules. |
| Protein Name | Lck-Interacting Transmembrane Adapter 1Lck-Interacting Membrane ProteinLck-Interacting Molecule |
| Cellular Localisation | Cell MembraneSingle-Pass Type Iii Membrane ProteinPresent In Lipid RaftsRecruited To The Immunological Synapse Upon Conjugation Of T-Cell With Antigen-Presenting Cell |
| Alternative Antibody Names | Anti-Lck-Interacting Transmembrane Adapter 1 antibodyAnti-Lck-Interacting Membrane Protein antibodyAnti-Lck-Interacting Molecule antibodyAnti-LIME1 antibodyAnti-LIME antibodyAnti-LP8067 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
