| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | Xrcc5 |
| Gene ID: | 22596 |
| Uniprot ID: | XRCC5_MOUSE |
| Immunogen Region: | 543-732 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 543-732 of mouse Ku80 (NP_033559.2). |
| Immunogen Sequence: | IKKKNQVTAQDVFQDNHEEG PAAKKYKTEKEEDHISISSL AEGNITKVGSVNPVENFRFL VRQKIASFEEASLQLISHIE QFLDTNETLYFMKSMDCIKA FREEAIQFSEEQRFNSFLEA LREKVEIKQLNHFWEIVVQD GVTLITKDEGPGSSITAEEA TKFLAPKDKAKEDTTGPEEA GDVDDLLDMI |
| Post Translational Modifications | ADP-ribosylated by PARP3. Phosphorylated on serine residues. Phosphorylation by PRKDC may enhance helicase activity. Sumoylated. Ubiquitinated by RNF8 via 'Lys-48'-linked ubiquitination following DNA damage, leading to its degradation and removal from DNA damage sites. Ubiquitinated by RNF138, leading to remove the Ku complex from DNA breaks. |
| Function | Single-stranded DNA-dependent ATP-dependent helicase that plays a key role in DNA non-homologous end joining (NHEJ) by recruiting DNA-PK to DNA. Required for double-strand break repair and V(D)J recombination. Also has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. During NHEJ, the XRCC5-XRRC6 dimer performs the recognition step: it recognizes and binds to the broken ends of the DNA and protects them from further resection. Binding to DNA may be mediated by XRCC6. The XRCC5-XRRC6 dimer acts as a regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5-XRRC6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. The XRCC5-XRRC6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5-XRRC6 dimer together with APEX1 acts as a negative regulator of transcription. In association with NAA15, the XRCC5-XRRC6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. As part of the DNA-PK complex, involved in the early steps of ribosome assembly by promoting the processing of precursor rRNA into mature 18S rRNA in the small-subunit processome. Binding to U3 small nucleolar RNA, recruits PRKDC and XRCC5/Ku86 to the small-subunit processome. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway. |
| Protein Name | X-Ray Repair Cross-Complementing Protein 5 Atp-Dependent Dna Helicase 2 Subunit 2 Atp-Dependent Dna Helicase Ii 80 Kda Subunit Ctc Box-Binding Factor 85 Kda Subunit Ctc85 Ctcbf Dna Repair Protein Xrcc5 Ku Autoantigen Protein P86 Homolog Ku80 Nuclear Factor Iv |
| Database Links | Reactome: R-MMU-5693571 Reactome: -MMU-6798695 |
| Cellular Localisation | Nucleus Nucleolus Chromosome |
| Alternative Antibody Names | Anti-X-Ray Repair Cross-Complementing Protein 5 antibody Anti-Atp-Dependent Dna Helicase 2 Subunit 2 antibody Anti-Atp-Dependent Dna Helicase Ii 80 Kda Subunit antibody Anti-Ctc Box-Binding Factor 85 Kda Subunit antibody Anti-Ctc85 antibody Anti-Ctcbf antibody Anti-Dna Repair Protein Xrcc5 antibody Anti-Ku Autoantigen Protein P86 Homolog antibody Anti-Ku80 antibody Anti-Nuclear Factor Iv antibody Anti-Xrcc5 antibody Anti-G22p2 antibody |
Information sourced from Uniprot.org