• IHC of paraffin-embedded human Spleen using anti-KAP1/TIF1 beta diluted 1/500-1/1000.
  • Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-KAP1/TIF1 beta mouse mAb (dilution 1:100).
  • Immunoprecipitation analysis of Hela cell lysates using KAP1/TIF1 beta mouse mAb.
  • Western blot detection of KAP1/TIF1 beta in 293T and HepG2 cell lysates using KAP1/TIF1 beta mouse mAb (1:1000 diluted).Observed band size: 110KDa.

Anti-KAP1/TIF Beta antibody [4E1-D12-F8] (STJ99057)

SKU:
STJ99057

Current Stock:
Host: Mouse
Applications: WB, ICC, IHC, IP
Reactivity: Human
Short Description: Mouse monoclonal anti-KAP1/TIF Beta antibody is suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry and Immunoprecipitation.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Monoclonal
Clone ID: 4E1-D12-F8
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG1
Dilution Range: WB 1:1000
ICC 1:100
Specificity: This antibody detects endogenous levels of KAP1/TIF1 beta and does not cross-react with related proteins.
Uniprot ID: TIF1B_HUMAN
Gene ID: 10155
Gene Symbol: TRIM28
Immunogen: Purified recombinant human KAP1/TIF1 beta protein fragments expressed in E.coli.
Post Translational Modifications ATM-induced phosphorylation on Ser-824 represses sumoylation leading to the de-repression of expression of a subset of genes involved in cell cycle control and apoptosis in response to genotoxic stress. Dephosphorylation by the phosphatases, PPP1CA and PP1CB forms, allows sumoylation and expression of TRIM28 target genes. Sumoylation/desumoylation events regulate TRIM28-mediated transcriptional repression. Sumoylation is required for interaction with CHD3 and SETDB1 and the corepressor activity. Represses and is repressed by Ser-824 phosphorylation. Enhances the TRIM28 corepressor activity, inhibiting transcriptional activity of a number of genes including GADD45A and CDKN1A/p21. Lys-554, Lys-779 and Lys-804 are the major sites of sumoylation. In response to Dox-induced DNA damage, enhanced phosphorylation on Ser-824 prevents sumoylation and allows de-repression of CDKN1A/p21. Auto-ubiquitinated.enhanced by MAGEA2 and MAGEC2. Citrullinated by PADI4.
Function Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. Also specifically sumoylates IRF7, thereby inhibiting its transactivation activity. Ubiquitinates p53/TP53 leading to its proteosomal degradation.the function is enhanced by MAGEC2 and MAGEA2, and possibly MAGEA3 and MAGEA6. Mediates the nuclear localization of KOX1, ZNF268 and ZNF300 transcription factors. In association with isoform 2 of ZFP90, is required for the transcriptional repressor activity of FOXP3 and the suppressive function of regulatory T-cells (Treg). Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells. Required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs). In ESCs, in collaboration with SETDB1, is also required for H3K9me3 and silencing of endogenous and introduced retroviruses in a DNA-methylation independent-pathway. Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing. The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions. Acts as a corepressor for ZFP568. (Microbial infection) Plays a critical role in the shutdown of lytic gene expression during the early stage of herpes virus 8 primary infection. This inhibition is mediated through interaction with herpes virus 8 protein LANA1.
Protein Name Transcription Intermediary Factor 1-Beta
Tif1-Beta
E3 Sumo-Protein Ligase Trim28
Krab-Associated Protein 1
Kap-1
Krab-Interacting Protein 1
Krip-1
Nuclear Corepressor Kap-1
Ring Finger Protein 96
Ring-Type E3 Ubiquitin Transferase Tif1-Beta
Tripartite Motif-Containing Protein 28
Database Links Reactome: R-HSA-212436
Reactome: R-HSA-3899300
Reactome: R-HSA-9609690
Cellular Localisation Nucleus
Associated With Centromeric Heterochromatin During Cell Differentiation Through Cbx1
Alternative Antibody Names Anti-Transcription Intermediary Factor 1-Beta antibody
Anti-Tif1-Beta antibody
Anti-E3 Sumo-Protein Ligase Trim28 antibody
Anti-Krab-Associated Protein 1 antibody
Anti-Kap-1 antibody
Anti-Krab-Interacting Protein 1 antibody
Anti-Krip-1 antibody
Anti-Nuclear Corepressor Kap-1 antibody
Anti-Ring Finger Protein 96 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Tif1-Beta antibody
Anti-Tripartite Motif-Containing Protein 28 antibody
Anti-TRIM28 antibody
Anti-KAP1 antibody
Anti-RNF96 antibody
Anti-TIF1B antibody

Information sourced from Uniprot.org

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