| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ITM2B |
| Gene ID: | 9445 |
| Uniprot ID: | ITM2B_HUMAN |
| Immunogen Region: | 81-266 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 81-266 of human ITM2B (NP_068839.1). |
| Immunogen Sequence: | LQPDDVYYCGIKYIKDDVIL NEPSADAPAALYQTIEENIK IFEEEEVEFISVPVPEFADS DPANIVHDFNKKLTAYLDLN LDKCYVIPLNTSIVMPPRNL LELLINIKAGTYLPQSYLIH EHMVITDRIENIDHLGFFIY RLCHDKETYKLQRRETIKGI QKREASNCFAIRHFENKFAV ETLICS |
| Tissue Specificity | Ubiquitous. Expressed in brain. |
| Post Translational Modifications | The ectodomain C-terminal part of the imBRI2 is processed by furin producing a secreted Bri23 peptide and a mature BRI2, membrane form (mBRI2). The remaining part of the ectodomain of mBRI2 containing the BRICHOS domain is cleaved by ADAM10 and is secreted (BRI2C, soluble form). The membrane-bound N-terminal fragment (BRI2C, membrane form) is further proteolytically processed by SPPL2A and SPPL2B through regulated intramembrane proteolysis producing a secreted C-peptide and a BRI2 intracellular domain (BRI2 ICD) released in the cytosol. Shedding by ADAM10 facilitates intramembrane cleavage but is not absolutely required for BRI2 ICD generation. Glycosylation at Asn-170 is important for cell surface localization, but doesn't affect furin- and ADAM10-induced proteolytic processing. |
| Function | Plays a regulatory role in the processing of the amyloid-beta A4 precursor protein (APP) and acts as an inhibitor of the amyloid-beta peptide aggregation and fibrils deposition. Plays a role in the induction of neurite outgrowth. Functions as a protease inhibitor by blocking access of secretases to APP cleavage sites. Mature BRI2 (mBRI2) functions as a modulator of the amyloid-beta A4 precursor protein (APP) processing leading to a strong reduction in the secretion of secretase-processed amyloid-beta protein 40 and amyloid-beta protein 42. Bri23 peptide prevents aggregation of APP amyloid-beta protein 42 into toxic oligomers. |
| Protein Name | Integral Membrane Protein 2b Immature Bri2 Imbri2 Protein E25b Transmembrane Protein Bri Bri Cleaved Into - Bri2 - Membrane Form Mature Bri2 Mbri2 - Bri2 Intracellular Domain Bri2 Icd - Bri2c - Soluble Form - Bri23 Peptide Bri2-23 Abri23 C-Terminal Peptide P23 Peptide |
| Database Links | Reactome: R-HSA-977225 |
| Cellular Localisation | Integral Membrane Protein 2b: Golgi Apparatus Membrane Single-Pass Type Ii Membrane Protein Immature Bri2 (Imbri2) Is Cleaved By Furin In The Golgi Into Mbri2 And A Bri23 Peptide Mbri2 Is Transported To The Plasma Membrane And Bri23 Peptide Is Secreted Bri2 Membrane Form: Cell Membrane Endosome Membrane Mature Bri2 (Mbri2) Needs To Be Transported From The Endoplasmic Reticulum Compartment To The Cell Membrane In Order To Be Able To Inhibit App Processing Bri23 Peptide: Secreted Detected In The Cerebral Spinal Fluid (Csf) Bri2c Soluble Form: Secreted |
| Alternative Antibody Names | Anti-Integral Membrane Protein 2b antibody Anti-Immature Bri2 antibody Anti-Imbri2 antibody Anti-Protein E25b antibody Anti-Transmembrane Protein Bri antibody Anti-Bri Cleaved Into - Bri2 - Membrane Form antibody Anti-Mature Bri2 antibody Anti-Mbri2 - Bri2 Intracellular Domain antibody Anti-Bri2 Icd - Bri2c - Soluble Form - Bri23 Peptide antibody Anti-Bri2-23 antibody Anti-Abri23 antibody Anti-C-Terminal Peptide antibody Anti-P23 Peptide antibody Anti-ITM2B antibody Anti-BRI antibody Anti-BRI2 antibody |
Information sourced from Uniprot.org