| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Rat/Pig |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Insulin receptor substrate 1 (603-652 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 IHC 1:100-1:300 IF 1:200-1:1000 ELISA 1:20000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | IRS1 |
| Gene ID: | 3667 |
| Uniprot ID: | IRS1_HUMAN |
| Immunogen Region: | 603-652 aa |
| Specificity: | IRS-1 Polyclonal Antibody detects endogenous levels of IRS-1 protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human IRS-1 at the amino acid range 603-652 |
| Function | Signaling adapter protein that participates in the signal transduction from two prominent receptor tyrosine kinases, insulin receptor/INSR and insulin-like growth factor I receptor/IGF1R. Plays therefore an important role in development, growth, glucose homeostasis as well as lipid metabolism. Upon phosphorylation by the insulin receptor, functions as a signaling scaffold that propagates insulin action through binding to SH2 domain-containing proteins including the p85 regulatory subunit of PI3K, NCK1, NCK2, GRB2 or SHP2. Recruitment of GRB2 leads to the activation of the guanine nucleotide exchange factor SOS1 which in turn triggers the Ras/Raf/MEK/MAPK signaling cascade. Activation of the PI3K/AKT pathway is responsible for most of insulin metabolic effects in the cell, and the Ras/Raf/MEK/MAPK is involved in the regulation of gene expression and in cooperation with the PI3K pathway regulates cell growth and differentiation. Acts a positive regulator of the Wnt/beta-catenin signaling pathway through suppression of DVL2 autophagy-mediated degradation leading to cell proliferation. |
| Protein Name | Insulin Receptor Substrate 1 Irs-1 |
| Database Links | Reactome: R-HSA-109704 Reactome: R-HSA-112399 Reactome: R-HSA-112412 Reactome: R-HSA-1257604 Reactome: R-HSA-1266695 Reactome: R-HSA-198203 Reactome: R-HSA-201556 Reactome: R-HSA-2219530 Reactome: R-HSA-2428928 Reactome: R-HSA-2586552 Reactome: R-HSA-5673001 Reactome: R-HSA-6811558 Reactome: R-HSA-74713 Reactome: R-HSA-74749 Reactome: R-HSA-9603381 Reactome: R-HSA-9725370 Reactome: R-HSA-982772 Reactome: R-HSA-9842663 |
| Cellular Localisation | Cytoplasm Nucleus Nuclear Or Cytoplasmic Localization Of Irs1 Correlates With The Transition From Proliferation To Chondrogenic Differentiation |
| Alternative Antibody Names | Anti-Insulin Receptor Substrate 1 antibody Anti-Irs-1 antibody Anti-IRS1 antibody |
Information sourced from Uniprot.org