• Immunohistochemistry analysis of INSM1 in paraffin-embedded human small cell lung cancer using INSM1 Rabbit polyclonal antibody (STJ11103303) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using INSM1 Rabbit polyclonal antibody (STJ11103303) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-INSM1 antibody (360-469) (STJ11103303)

SKU:
STJ11103303

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-INSM1 (360-469) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: INSM1
Gene ID: 3642
Uniprot ID: INSM1_HUMAN
Immunogen Region: 360-469
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 360-469 of human INSM1 (NP_002187.1).
Immunogen Sequence: SGSEDGLYECHHCAKKFRRQ AYLRKHLLAHHQALQAKGAP LAPPAEDLLALYPGPDEKAP QEAAGDGEGAGVLGLSASAE CHLCPVCGESFASKGAQERH LRLLHAAQVF
Tissue Specificity Expressed in pancreatic duct cells. Expressed in several tumor cell lines of neuroendocrine origin including pheochromocytoma, medullary thyroid carcinoma, insulinoma, medulloblastoma, retinoblastoma, pheochromacytoma, medullary thyroid carcinoma and small cell lung carcinoma.
Function Sequence-specific DNA-binding transcriptional regulator that plays a key role in neurogenesis and neuroendocrine cell differentiation during embryonic and/or fetal development. Binds to the consensus sequence 5'-TGTCTCTTGAGGGCGA-3' in target promoters. Acts as a transcriptional repressor of NEUROD1 and INS expression via its interaction with cyclin CCND1 in a cell cycle-independent manner. Negatively regulates skeletal muscle-specific gene expression in endocrine cells of the pituitary by inhibiting the Notch signaling pathway. Represses target gene transcription by recruiting chromatin-modifying factors, such as HDAC1, HDAC2, HDAC3, KDM1A and RCOR1 histone deacetylases. Binds to its own promoter, suggesting autoregulation as a self-control feedback mechanism. Competes with histone H3 for the same binding site on the histone demethylase complex formed by KDM1A and RCOR1, and thereby inhibits demethylation of histone H3 at 'Lys-4'. Promotes the generation and expansion of neuronal basal progenitor cells in the developing neocortex. Involved in the differentiation of endocrine cells of the developing anterior pituitary gland, of the pancreas and intestine, and of sympatho-adrenal cells in the peripheral nervous system. Promotes cell cycle signaling arrest and inhibition of cellular proliferation.
Protein Name Insulinoma-Associated Protein 1
Zinc Finger Protein Ia-1
Database Links Reactome: R-HSA-210746
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Insulinoma-Associated Protein 1 antibody
Anti-Zinc Finger Protein Ia-1 antibody
Anti-INSM1 antibody
Anti-IA1 antibody

Information sourced from Uniprot.org

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