• Immunofluorescence analysis of paraffin-embedded mouse small intestine using IFN-beta Rabbit polyclonal antibody (STJ11105175) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HepG2 cells using IFN-beta Rabbit polyclonal antibody (STJ11105175) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of recombinant Mouse IFN-beta Protein using IFN-beta Rabbit polyclonal antibody (STJ11105175) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 10ng/5ng/1ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-IFN-beta antibody (1-182) (STJ11105175)

SKU:
STJ11105175

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IFN-beta (1-182) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Ifnb1
Gene ID: 15977
Uniprot ID: IFNB_MOUSE
Immunogen Region: 1-182
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-182 of mouse IFN-beta (NP_034640.1)
Immunogen Sequence: MNNRWILHAAFLLCFSTTAL SINYKQLQLQERTNIRKCQE LLEQLNGKINLTYRADFKIP MEMTEKMQKSYTAFAIQEML QNVFLVFRNNFSSTGWNETI VVRLLDELHQQTVFLKTVLE EKQEERLTWEMSSTALHLKS YYWRVQRYLKLMKYNSYAWM VVRAEIFRNFLIIRRLTRNF QN
Post Translational Modifications This beta interferon does not have a disulfide bond.
Function Type I interferon cytokine that plays a key role in the innate immune response to infection, developing tumors and other inflammatory stimuli. Signals via binding to high-affinity (IFNAR2) and low-affinity (IFNAR1) heterodimeric receptor, activating the canonical Jak-STAT signaling pathway resulting in transcriptional activation or repression of interferon-regulated genes that encode the effectors of the interferon response, such as antiviral proteins, regulators of cell proliferation and differentiation, and immunoregulatory proteins. Signals mostly via binding to a IFNAR1-IFNAR2 heterodimeric receptor, but can also function with IFNAR1 alone and independently of Jak-STAT pathways. Elicits a wide variety of responses, including antiviral and antibacterial activities, and can regulate the development of B-cells, myelopoiesis and lipopolysaccharide (LPS)-inducible production of tumor necrosis factor. Plays a role in neuronal homeostasis by regulating dopamine turnover and protecting dopaminergic neurons: acts by promoting neuronal autophagy and alpha-synuclein clearance, thereby preventing dopaminergic neuron loss. IFNB1 is more potent than interferon-alpha (IFN-alpha) in inducing the apoptotic and antiproliferative pathways required for control of tumor cell growth.
Protein Name Interferon Beta
Ifn-Beta
Database Links Reactome: R-MMU-909733
Reactome: -MMU-912694
Cellular Localisation Secreted
Alternative Antibody Names Anti-Interferon Beta antibody
Anti-Ifn-Beta antibody
Anti-Ifnb1 antibody
Anti-Ifb antibody
Anti-Ifnb antibody

Information sourced from Uniprot.org

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