| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IHC-P:1:50-1:200 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | IFIH1 |
| Gene ID: | 64135 |
| Uniprot ID: | IFIH1_HUMAN |
| Immunogen Region: | 1-1025 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-205 of human IFIH1 (NP_071451.2). |
| Immunogen Sequence: | MSNGYSTDENFRYLISCFRA RVKMYIQVEPVLDYLTFLPA EVKEQIQRTVATSGNMQAVE LLLSTLEKGVWHLGWTREFV EALRRTGSPLAARYMNPELT DLPSPSFENAHDEYLQLLNL LQPTLVDKLLVRDVLDKCME EELLTIEDRNRIAAAENNGN ESGVRELLKRIVQKENWFSA FLNVLRQTGNNELVQELTGS DCSES |
| Tissue Specificity | Widely expressed, at a low level. Expression is detected at slightly highest levels in placenta, pancreas and spleen and at barely levels in detectable brain, testis and lung. |
| Post Translational Modifications | Sumoylated. Sumoylation positively regulates its role in type I interferon induction and is enhanced by PIAS2-beta. Ubiquitinated by RNF125, leading to its degradation by the proteasome. USP17/UPS17L2-dependent deubiquitination positively regulates the receptor. Ubiquitinated by TRIM25 via 'Lys-63'-linked ubiquitination, promoting activation of IFIH1/MDA5. Ubiquitinated by TRIM40 via 'Lys-48'-linked ubiquitination.leading to proteasomal degradation. Ubiquitinated by TRIM65 via 'Lys-63'-linked ubiquitination, promoting activation of IFIH1/MDA5. ISGylated by ISG15. ISGylation increases upon infection with dengue (DENV) or Zika (ZIKV) viruses. ISGylation at Lys-23 and Lys-43 is dependent of dephosphorylation at Ser-88, regulates mitochondrial translocation and oligomerization. Essential for IFIH1/MDA5-mediated cytokine responses and restriction of virus replication. Phosphorylated at Ser-88. Dephosphorylated by phosphatases PPP11CA/PPP11CC.dephosphorylation precedes and is required for ISGylation. During apoptosis, processed into 3 cleavage products. The helicase-containing fragment, once liberated from the CARD domains, translocate from the cytoplasm to the nucleus. The processed protein significantly sensitizes cells to DNA degradation. (Microbial infection) Cleaved and inactivated by the protease 2A of coxsackievirus B3, poliovirus and enterovirus 71 allowing the virus to disrupt the host type I interferon production. |
| Function | Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and pro-inflammatory cytokines. Its ligands include mRNA lacking 2'-O-methylation at their 5' cap and long-dsRNA (>1 kb in length). Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs).IFN-alpha and IFN-beta. Responsible for detecting the Picornaviridae family members such as encephalomyocarditis virus (EMCV), mengo encephalomyocarditis virus (ENMG), and rhinovirus. Detects coronavirus SARS-CoV-2. Can also detect other viruses such as dengue virus (DENV), west Nile virus (WNV), and reovirus. Also involved in antiviral signaling in response to viruses containing a dsDNA genome, such as vaccinia virus. Plays an important role in amplifying innate immune signaling through recognition of RNA metabolites that are produced during virus infection by ribonuclease L (RNase L). May play an important role in enhancing natural killer cell function and may be involved in growth inhibition and apoptosis in several tumor cell lines. |
| Protein Name | Interferon-Induced Helicase C Domain-Containing Protein 1 Clinically Amyopathic Dermatomyositis Autoantigen 140 Kda Cadm-140 Autoantigen Helicase With 2 Card Domains Helicard Interferon-Induced With Helicase C Domain Protein 1 Melanoma Differentiation-Associated Protein 5 Mda-5 Murabutide Down-Regulated Protein Rig-I-Like Receptor 2 Rlr-2 Rna Helicase-Dead Box Protein 116 |
| Database Links | Reactome: R-HSA-168928 Reactome: R-HSA-5689880 Reactome: R-HSA-5689896 Reactome: R-HSA-918233 Reactome: R-HSA-933541 Reactome: R-HSA-933542 Reactome: R-HSA-933543 Reactome: R-HSA-936440 Reactome: R-HSA-9692916 Reactome: R-HSA-9705671 Reactome: R-HSA-9833109 |
| Cellular Localisation | Cytoplasm Nucleus Mitochondrion Upon Viral Rna Stimulation And Isgylation Translocates From Cytosol To Mitochondrion May Be Found In The Nucleus During Apoptosis |
| Alternative Antibody Names | Anti-Interferon-Induced Helicase C Domain-Containing Protein 1 antibody Anti-Clinically Amyopathic Dermatomyositis Autoantigen 140 Kda antibody Anti-Cadm-140 Autoantigen antibody Anti-Helicase With 2 Card Domains antibody Anti-Helicard antibody Anti-Interferon-Induced With Helicase C Domain Protein 1 antibody Anti-Melanoma Differentiation-Associated Protein 5 antibody Anti-Mda-5 antibody Anti-Murabutide Down-Regulated Protein antibody Anti-Rig-I-Like Receptor 2 antibody Anti-Rlr-2 antibody Anti-Rna Helicase-Dead Box Protein 116 antibody Anti-IFIH1 antibody Anti-MDA5 antibody Anti-RH116 antibody |
Information sourced from Uniprot.org