| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-HOOK1 (489-728) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
HOOK1 |
| Gene ID: |
51361 |
| Uniprot ID: |
HOOK1_HUMAN |
| Immunogen Region: |
489-728 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 489-728 of human HOOK1 (NP_056972.1). |
| Immunogen Sequence: |
IEELQEQLEQKHRKMNELET EQRLSKERIRELQQQIEDLQ KSLQEQGSKSEGESSSKLKQ KLEAHMEKLTEVHEELQKKQ ELIEDLQPDINQNVQKINEL EAALQKKDEDMKAMEERYKM YLEKARNVIKTLDPKLNPAS AEIMLLRKQLAEKERRIEIL ESECKVAKFRDYEEKLIVSA WYNKSLAFQKLGMESRLVSG GGACSDTGACTPARSFLAQQ RHITNTRRNLSVKVPATTS |
| Function | Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). FHF complex promotes the distribution of AP-4 complex to the perinuclear area of the cell. Required for spermatid differentiation. Probably involved in the positioning of the microtubules of the manchette and the flagellum in relation to the membrane skeleton. |
| Protein Name | Protein Hook Homolog 1H-Hook1Hhk1 |
| Cellular Localisation | CytoplasmCytoskeletonLocalizes To Punctate Cytoplasmic Foci Which Do Not Appear To Overlap With Early Or Late EndosomesThe Endoplasmic ReticulumMultivesicular Bodies (Mvbs)LysosomesOr MitochondriaOften Found In Close Association With MicrotubulesDoes Not Associate With The Golgi ComplexDuring SpermiogenesisIt Localizes To The Manchette In Spermatids From Steps 8-10It Is Also Present Between The Microtubule Manchette And The NucleusDuring Manchette ElongationIt Is Preferentially Localized To The Nuclear Ring Of The ManchetteWhereas The Strong Localization To The Manchette DecreasesIn More Mature SpermatidsWhile The Manchette Migrates PosteriorlyIt Localizes To Punctuates SpotsAt Later Stages Of Spermatid DifferentiationThe Punctuate Expression Pattern Is Found At Both The Attachment Site And The Proximal End Of The Elongated ManchetteIn ContrastIt Is Not Present In Mature Spermatozoa |
| Alternative Antibody Names | Anti-Protein Hook Homolog 1 antibodyAnti-H-Hook1 antibodyAnti-Hhk1 antibodyAnti-HOOK1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and IPO4 knockout (KO) 293T cells, using [KO Validated] IPO4 Rabbit polyclonal antibody (STJ11100778) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89714/358196/STJ11100778_1__20770.1713122514.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and IPO4 knockout (KO) 293T cells, using [KO Validated] IPO4 Rabbit polyclonal antibody (STJ11100778) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")