| Short Description : | Rabbit polyclonal HOOK1 (489-728) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | HOOK1 |
| Gene ID: | 51361 |
| Uniprot ID: | HOOK1_HUMAN |
| Immunogen Region: | 489-728 |
| Immunogen Sequence: | IEELQEQLEQKHRKMNELET EQRLSKERIRELQQQIEDLQ KSLQEQGSKSEGESSSKLKQ KLEAHMEKLTEVHEELQKKQ ELIEDLQPDINQNVQKINEL EAALQKKDEDMKAMEERYKM YLEKARNVIKTLDPKLNPAS AEIMLLRKQLAEKERRIEIL ESECKVAKFRDYEEKLIVSA WYNKSLAFQKLGMESRLVSG GGACSDTGACTPARSFLAQQ RHITNTRRNLSVKVPATTS |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 489-728 of human HOOK1 (NP_056972.1). |
| Function | Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). FHF complex promotes the distribution of AP-4 complex to the perinuclear area of the cell. Required for spermatid differentiation. Probably involved in the positioning of the microtubules of the manchette and the flagellum in relation to the membrane skeleton. |
| Protein Name | Protein Hook Homolog 1H-Hook1Hhk1 |
| Database Links | |
| Cellular Localisation | CytoplasmCytoskeletonLocalizes To Punctate Cytoplasmic Foci Which Do Not Appear To Overlap With Early Or Late EndosomesThe Endoplasmic ReticulumMultivesicular Bodies (Mvbs)LysosomesOr MitochondriaOften Found In Close Association With MicrotubulesDoes Not Associate With The Golgi ComplexDuring SpermiogenesisIt Localizes To The Manchette In Spermatids From Steps 8-10It Is Also Present Between The Microtubule Manchette And The NucleusDuring Manchette ElongationIt Is Preferentially Localized To The Nuclear Ring Of The ManchetteWhereas The Strong Localization To The Manchette DecreasesIn More Mature SpermatidsWhile The Manchette Migrates PosteriorlyIt Localizes To Punctuates SpotsAt Later Stages Of Spermatid DifferentiationThe Punctuate Expression Pattern Is Found At Both The Attachment Site And The Proximal End Of The Elongated ManchetteIn ContrastIt Is Not Present In Mature Spermatozoa |
| Alternative Antibody Names | Anti-Protein Hook Homolog 1 antibodyAnti-H-Hook1 antibodyAnti-Hhk1 antibodyAnti-HOOK1 antibody |
Information sourced from Uniprot.org
![Anti-MLH1 antibody [G168-728] (STJ16100394)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/149111/346732/STJ16100394_1__48360.1681998822.jpg?c=1 "Anti-MLH1 antibody [G168-728] (STJ16100394)")
