Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-HDGF antibody (141-190 aa) (STJ98794)

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STJ98794
£48.00 - £252.00
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Host: Rabbit
Applications: IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description : Rabbit polyclonal antibody anti-Hepatoma-derived growth factor (141-190 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC-P 1:50-200
ELISA 1:10000-20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HDGF
Gene ID: 3068
Uniprot ID: HDGF_HUMAN
Immunogen Region: 141-190 aa
Specificity: The antibody detects endogenous HDGF
Immunogen: Synthetic peptide from the human protein at the amino acid range 141-190
Post Translational Modifications Sumoylated with SUMO1. Sumoylation prevents binding to chromatin. Phosphorylation at Ser-165 is likely to be required for secretion.
Function Isoform 1: Acts as a transcriptional repressor. Has mitogenic activity for fibroblasts. Heparin-binding protein. Isoform 2: Does not have mitogenic activity for fibroblasts. Does not bind heparin. Isoform 3: Has mitogenic activity for fibroblasts. Heparin-binding protein.
Protein Name Hepatoma-Derived Growth Factor
Hdgf
High Mobility Group Protein 1-Like 2
Hmg-1l2
Database Links Reactome: R-HSA-381038
Cellular Localisation Isoform 1: Nucleus
Cytoplasm
Secreted
Extracellular Exosome
Secreted By Exosomes And Is Located Inside The Exosome
May Also Be Secreted As Free Protein Via An As Yet Unknown Pathway
Isoform 2: Nucleus
Secreted By Exosomes And Is Located On The Outer Exosome Surface
Isoform 3: Nucleus
Alternative Antibody Names Anti-Hepatoma-Derived Growth Factor antibody
Anti-Hdgf antibody
Anti-High Mobility Group Protein 1-Like 2 antibody
Anti-Hmg-1l2 antibody
Anti-HDGF antibody
Anti-HMG1L2 antibody

Information sourced from Uniprot.org

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