| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Histone deacetylase 5 (464-513 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 IHC 1:100-1:300 IF 1:200-1:1000 ELISA 1:20000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | HDAC5 |
| Gene ID: | 10014 |
| Uniprot ID: | HDAC5_HUMAN |
| Immunogen Region: | 464-513 aa |
| Specificity: | HDAC5 Polyclonal Antibody detects endogenous levels of HDAC5 protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human HDAC5 at the amino acid range 464-513 |
| Post Translational Modifications | Phosphorylated by AMPK, CaMK1, SIK1 and PRKD1 at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm and inhibition. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import. Phosphorylated by GRK5, leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription. Ubiquitinated. Polyubiquitination however does not lead to its degradation. |
| Function | Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Serves as a corepressor of RARA and causes its deacetylation. In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response. |
| Protein Name | Histone Deacetylase 5 Hd5 Antigen Ny-Co-9 |
| Database Links | Reactome: R-HSA-2122947 Reactome: R-HSA-2644606 Reactome: R-HSA-2894862 Reactome: R-HSA-350054 Reactome: R-HSA-8943724 |
| Cellular Localisation | Nucleus Cytoplasm Shuttles Between The Nucleus And The Cytoplasm In Muscle Cells It Shuttles Into The Cytoplasm During Myocyte Differentiation The Export To Cytoplasm Depends On The Interaction With A 14-3-3 Chaperone Protein And Is Due To Its Phosphorylation At Ser-259 And Ser-498 By Ampk Camk1 And Sik1 |
| Alternative Antibody Names | Anti-Histone Deacetylase 5 antibody Anti-Hd5 antibody Anti-Antigen Ny-Co-9 antibody Anti-HDAC5 antibody Anti-KIAA0600 antibody |
Information sourced from Uniprot.org