• Western blot analysis of various lysates using HAUS1 Rabbit polyclonal antibody (STJ119361) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HAUS1 antibody (120-250) (STJ119361)

SKU:
STJ119361

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HAUS1 (120-250) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HAUS1
Gene ID: 115106
Uniprot ID: HAUS1_HUMAN
Immunogen Region: 120-250
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 120-250 of human HAUS1 (NP_612452.1).
Immunogen Sequence: NDLTSDLFRTKSKSEEIKIE LEKLEKNLTATLVLEKCLQE DVKKAELHLSTERAKVDNRR QNMDFLKAKSEEFRFGIKAA EEQLSARGMDASLSHQSLVA LSEKLARLKQQTIPLKKKLE SYLDLMPNPSL
Tissue Specificity Widely expressed. Expressed in pancreas, kidney, skeletal muscle, liver and heart. Weakly expressed in lung, brain and placenta.
Function Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex.
Protein Name Haus Augmin-Like Complex Subunit 1
Coiled-Coil Domain-Containing Protein 5
Enhancer Of Invasion-Cluster
Hei-C
Database Links Reactome: R-HSA-2565942
Reactome: R-HSA-380259
Reactome: R-HSA-380270
Reactome: R-HSA-380284
Reactome: R-HSA-380320
Reactome: R-HSA-5620912
Reactome: R-HSA-8854518
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Spindle
Spindle Pole
Localizes With The Spindle Poles In Mitotic Cells
In Metaphase
Localizes To The Mitotic Asters And Is Highly Punctate On The Microtubule Array
During Later Stages Of Mitosis
Remains On The Spindle But Is Not Present At The Interzone
And Is Finally Observed At The Microtubule Bundles Proximal To The Midbody
Clearly Excluded From The Midbody
In Contrast
Does Not Colocalize With The Tubulin Cytoskeleton In Interphase Cells
In Interphase
Localized At The Centrosome And Diffusely In The Cytoplasm
Localizes To Mitotic Spindle Microtubules
Alternative Antibody Names Anti-Haus Augmin-Like Complex Subunit 1 antibody
Anti-Coiled-Coil Domain-Containing Protein 5 antibody
Anti-Enhancer Of Invasion-Cluster antibody
Anti-Hei-C antibody
Anti-HAUS1 antibody
Anti-CCDC5 antibody
Anti-HEIC antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance