| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | GRN |
| Gene ID: | 2896 |
| Uniprot ID: | GRN_HUMAN |
| Immunogen Region: | 20-593 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-310 of human Granulin (NP_002078.1). |
| Immunogen Sequence: | CPDGQFCPVACCLDPGGASY SCCRPLLDKWPTTLSRHLGG PCQVDAHCSAGHSCIFTVSG TSSCCPFPEAVACGDGHHCC PRGFHCSADGRSCFQRSGNN SVGAIQCPDSQFECPDFSTC CVMVDGSWGCCPMPQASCCE DRVHCCPHGAFCDLVHTRCI TPTGTHPLAKKLPAQRTNRA VALSSSVMCPDARSRCPDGS TCCELPSGKYGCCPMPNATC CSDHLHCCPQDTVCDLIQS |
| Tissue Specificity | In myelogenous leukemic cell lines of promonocytic, promyelocytic, and proerythroid lineage, in fibroblasts, and very strongly in epithelial cell lines. Present in inflammatory cells and bone marrow. Highest levels in kidney. |
| Post Translational Modifications | Cleaved by ELANE.proteolysis is blocked by SLPI and is concentration- and time-dependent and induces CXCL8/IL-8 production.granulin-3 and granulin-4 are resistant to ELANE. Cleaved by CTSL in lysosome thus regulating the maturation and turnover of progranulin within the lysosome. |
| Function | Secreted protein that acts as a key regulator of lysosomal function and as a growth factor involved in inflammation, wound healing and cell proliferation. Regulates protein trafficking to lysosomes and, also the activity of lysosomal enzymes. Facilitates also the acidification of lysosomes, causing degradation of mature CTSD by CTSB. In addition, functions as a wound-related growth factor that acts directly on dermal fibroblasts and endothelial cells to promote division, migration and the formation of capillary-like tubule structures. Also promotes epithelial cell proliferation by blocking TNF-mediated neutrophil activation preventing release of oxidants and proteases. Moreover, modulates inflammation in neurons by preserving neurons survival, axonal outgrowth and neuronal integrity. Granulin-4: Promotes proliferation of the epithelial cell line A431 in culture. Granulin-3: Inhibits epithelial cell proliferation and induces epithelial cells to secrete IL-8. Granulin-7: Stabilizes CTSD through interaction with CTSD leading to maintain its aspartic-type peptidase activity. |
| Protein Name | Progranulin Pgrn Acrogranin Epithelin Precursor Glycoprotein Of 88 Kda Gp88 Glycoprotein 88 Granulin Precursor Pc Cell-Derived Growth Factor Pcdgf Proepithelin Pepi Cleaved Into - Paragranulin - Granulin-1 Granulin G - Granulin-2 Granulin F - Granulin-3 Epithelin-2 Granulin B - Granulin-4 Epithelin-1 Granulin A - Granulin-5 Granulin C - Granulin-6 Granulin D - Granulin-7 Granulin E |
| Database Links | Reactome: R-HSA-6798695 |
| Cellular Localisation | Secreted Lysosome Endocytosed By Sort1 And Delivred To Lysosomes Targeted To Lysosome By Psap Via M6pr And Lrp1 In Both Biosynthetic And Endocytic Pathways Co-Localized With Gba1 In The Intracellular Trafficking Compartments Until To Lysosome |
| Alternative Antibody Names | Anti-Progranulin antibody Anti-Pgrn antibody Anti-Acrogranin antibody Anti-Epithelin Precursor antibody Anti-Glycoprotein Of 88 Kda antibody Anti-Gp88 antibody Anti-Glycoprotein 88 antibody Anti-Granulin Precursor antibody Anti-Pc Cell-Derived Growth Factor antibody Anti-Pcdgf antibody Anti-Proepithelin antibody Anti-Pepi Cleaved Into - Paragranulin - Granulin-1 antibody Anti-Granulin G - Granulin-2 antibody Anti-Granulin F - Granulin-3 antibody Anti-Epithelin-2 antibody Anti-Granulin B - Granulin-4 antibody Anti-Epithelin-1 antibody Anti-Granulin A - Granulin-5 antibody Anti-Granulin C - Granulin-6 antibody Anti-Granulin D - Granulin-7 antibody Anti-Granulin E antibody Anti-GRN antibody |
Information sourced from Uniprot.org