• Western blot analysis of various lysates using GOLPH4 Rabbit polyclonal antibody (STJ116688) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-GOLIM4 antibody (34-300) (STJ116688)

SKU:
STJ116688

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GOLIM4 (34-300) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GOLIM4
Gene ID: 27333
Uniprot ID: GOLI4_HUMAN
Immunogen Region: 34-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 34-300 of human GOLPH4 (NP_055313.1).
Immunogen Sequence: YELQTQLRKAEAVALKYQQH QESLSAQLQVVYEHRSRLEK SLQKERLEHKKAKEDFLVYK LEAQETLNKGRQDSNSRYSA LNVQHQMLKSQHEELKKQHS DLEEEHRKQGEDFSRTFNDH KQKYLQLQQEKEQELSKLKE TVYNLREENRQLRKAHQDIH TQLQDVKQQHKNLLSEHEQL VVTLEDHKSALAAAQTQVAE YKQLKDTLNRIPSLRKPDPA EQQNVTQVAHSPQGYNTAR
Post Translational Modifications Phosphorylated probably by c-AMP-dependent kinases in its lumenal part. O-glycosylated.modified by sialic acid residues. N-glycosylated.N-glycans are probably of the complex type and modified by sialic acid residues.
Function Plays a role in endosome to Golgi protein trafficking.mediates protein transport along the late endosome-bypass pathway from the early endosome to the Golgi.
Protein Name Golgi Integral Membrane Protein 4
Golgi Integral Membrane Protein - Cis
Gimpc
Golgi Phosphoprotein 4
Golgi-Localized Phosphoprotein Of 130 Kda
Golgi Phosphoprotein Of 130 Kda
Database Links Reactome: R-HSA-6811438
Cellular Localisation Golgi Apparatus
Golgi Stack Membrane
Single-Pass Type Ii Membrane Protein
Endosome Membrane
Membrane
Lipid-Anchor
Localizes To Cis And Medial Golgi Cisternae
Probably Cycles Between Early Golgi And Distal Compartments Like Endosome
Alternative Antibody Names Anti-Golgi Integral Membrane Protein 4 antibody
Anti-Golgi Integral Membrane Protein - Cis antibody
Anti-Gimpc antibody
Anti-Golgi Phosphoprotein 4 antibody
Anti-Golgi-Localized Phosphoprotein Of 130 Kda antibody
Anti-Golgi Phosphoprotein Of 130 Kda antibody
Anti-GOLIM4 antibody
Anti-GIMPC antibody
Anti-GOLPH4 antibody
Anti-GPP130 antibody

Information sourced from Uniprot.org

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