• Western blot analysis of extracts of various cell lines, using GNAO1 antibody (STJ23814) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST.
  • Immunofluorescence analysis of mouse brain cells using GNAO1 rabbit polyclonal antibody (STJ23814) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat brain cells using GNAO1 rabbit polyclonal antibody (STJ23814) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-GNAO1 antibody (60-180) (STJ23814)

SKU:
STJ23814

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GNAO1
Gene ID: 2775
Uniprot ID: GNAO_HUMAN
Immunogen Region: 60-180
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 60-180 of human GNAO1 (NP_066268.1).
Immunogen Sequence: GFSGEDVKQYKPVVYSNTIQ SLAAIVRAMDTLGIEYGDKE RKADAKMVCDVVSRMEDTEP FSAELLSAMMRLWGDSGIQE CFNRSREYQLNDSAKYYLDS LDRIGAADYQPTEQDILRTR V
Post Translational Modifications Histaminylated at Gln-205 residues by TGM2.
Function Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins. Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels.
Protein Name Guanine Nucleotide-Binding Protein G(O Subunit Alpha
Database Links Reactome: R-HSA-4086398
Cellular Localisation Cell Membrane
Membrane
Lipid-Anchor
Alternative Antibody Names Anti-Guanine Nucleotide-Binding Protein G(O Subunit Alpha antibody
Anti-GNAO1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance