• Immunohistochemistry analysis of paraffin-embedded mouse brain using GM13125 antibody (STJ114493) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human stomach using GM13125 antibody (STJ114493) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using GM13125 antibody (STJ114493) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Gm13125 antibody (STJ114493) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-Gm13125 antibody (1-477) (STJ114493)

SKU:
STJ114493

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Gm13125 (1-477) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRAMEF20
Gene ID: 645425
Uniprot ID: PRA20_HUMAN
Immunogen Region: 1-477
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-477 of mouse Gm13125 (NP_001108549.1).
Immunogen Sequence: MSSKPLLTLQELAAQNILRN EALAISALQYLPELFLPQLL KQAYDGNHLKVLRAIVSSWP FPRLPLGALKKRTPYLETQL QVVLEEVDKLLIQEVHPREY KLEVLDLRSVGQNYLNVWPG AMDDWLPKTTQTDPCCSKTV MTQPLKVLVDLQLNNRGQSR LFSYLVQWSDRRKGLLQLYC NKLQIWSPSQQNYRKLSQKV NLDYVETLGLHSSCSPSFLL NFAPYLRQMRNLRKLALSN
Protein Name Prame Family Member 20
Alternative Antibody Names Anti-Prame Family Member 20 antibody
Anti-PRAMEF20 antibody
Anti-PRAMEF21 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance