| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
GHR |
| Gene ID: |
2690 |
| Uniprot ID: |
GHR_HUMAN |
| Immunogen Region: |
26-260 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 26-260 of human GHR (NP_001229328.1). |
| Immunogen Sequence: |
FSGSEATAAILSRAPWSLQS VNPGLKTNSSKEPKFTKCRS PERETFSCHWTDEVHHGTKN LGPIQLFYTRRNTQEWTQEW KECPDYVSAGENSCYFNSSF TSIWIPYCIKLTSNGGTVDE KCFSVDEIVQPDPPIALNWT LLNVSLTGIHADIQVRWEAP RNADIQKGWMVLEYELQYKE VNETKWKMMDPILTTSVPVY SLKVDKEYEVRVRSKQRNSG NYGEFSEVLYVTLPQ |
| Tissue Specificity | Expressed in various tissues with high expression in liver and skeletal muscle. Isoform 2: Isoform 2 is expressed in lung, stomach and muscle. Isoform 4: Predominantly expressed in kidney, bladder, adrenal gland and brain stem. Highly expressed in placental villi. |
| Post Translational Modifications | The soluble form (GHBP) is produced by phorbol ester-promoted proteolytic cleavage at the cell surface (shedding) by ADAM17/TACE. Shedding is inhibited by growth hormone (GH) binding to the receptor probably due to a conformational change in GHR rendering the receptor inaccessible to ADAM17. On GH binding, phosphorylated on tyrosine residues in the cytoplasmic domain by JAK2. Ubiquitinated by the ECS(SOCS2) complex following ligand-binding and phosphorylation by JAK2, leading to its degradation by the proteasome. Regulation by the ECS(SOCS2) complex acts as a negative feedback loop of growth hormone receptor signaling. Ubiquitination is not sufficient for GHR internalization. |
| Function | Receptor for pituitary gland growth hormone (GH1) involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway. Growth hormone-binding protein: The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling. Isoform 2: Up-regulates the production of the soluble Growth hormone-binding protein form (GHBP) and acts as a negative inhibitor of growth hormone signaling. |
| Protein Name | Growth Hormone ReceptorGh ReceptorSomatotropin Receptor Cleaved Into - Growth Hormone-Binding ProteinGh-Binding ProteinGhbpSerum-Binding Protein |
| Database Links | Reactome: R-HSA-1170546Reactome: R-HSA-982772 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinOn Growth Hormone BindingGhr Is UbiquitinatedInternalizedDown-Regulated And Transported Into A Degradative Or Non-Degradative PathwayIsoform 2: Cell MembraneRemains Fixed To The Cell Membrane And Is Not InternalizedGrowth Hormone-Binding Protein: SecretedComplexed To A Substantial Fraction Of Circulating Gh |
| Alternative Antibody Names | Anti-Growth Hormone Receptor antibodyAnti-Gh Receptor antibodyAnti-Somatotropin Receptor Cleaved Into - Growth Hormone-Binding Protein antibodyAnti-Gh-Binding Protein antibodyAnti-Ghbp antibodyAnti-Serum-Binding Protein antibodyAnti-GHR antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
