• Immunofluorescence of a section of mouse hippocampus colabeled with Anti-GFAP (STJA0003659, green, 1:5000) and Anti-FOX3 (red). The Anti-FOX3 labels the nuclei and proximal perikarya of neurons while the Anti-GFAP labels a network of astroglial cells. The blue is DAPI staining of nuclear DNA.
  • Western blot of rat cortical lysate showing specific immunolabeling of the ~50 kDa GFAP protein.
  • Immunofluorescence of a section of mouse prefrontal cortex labeled with Anti-GFAP (STJA0003659, red, 1:1000). Image courtesy Andrea Cardenas, Rosalind Franklin Unversity, Medicine and Science.
  • Immunolabeling of mixed neuron and glia cultures where astrocytes are strongly and specifically labeled with Anti-GFAP (STJA0003659, 1:1000, red) , and nuclear staining with DAPI (blue).

Anti-GFAP antibody (STJA0003659)

SKU:
STJA0003659-100

£394.50
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Host: Chicken
Applications: WB/IHC/ICC
Reactivity: Bovine/Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Chicken polyclonal antibody anti-GFAP is suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgY
Formulation: Total IgY fraction in PBS + 10 mM Sodium Azide.
Purification: This antibody was total igy fraction.
Dilution Range: WB 1:10, 000
IHC 1:1000-1:5000
ICC 1:1000-1:5000
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: Recombinant and purification bovine GFAP.
Background Glial Fibrillary Acidic Protein (GFAP) was discovered by Amico Bignami and co-workers as a major fibrous protein of multiple sclerosis plaques (Bignami at al., 1972). It was subsequently found to be a member of the 10nm or intermediate filament (IF) family, specifically the IF family Class III, which also includes peripherin, desmin and vimentin. GFAP is strongly and specifically expressed in astrocytes and certain other astroglia in the CNS, in satellite cells, peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In many damage and disease states GFAP expression is heavily upregulated in astrocytes. In addition, neural stem cells frequently strongly express GFAP. Point mutations in the protein coding region of the GFAP gene lead to Alexander disease which is characterized by the presence of abnormal astrocytes containing GFAP protein aggregates known as Rosenthal fibers (Brenner et al., 2001). Western blot of rat cortical lysate showing specific immunolabeling of the ~50 kDa GFAP protein.

Information sourced from Uniprot.org

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