| Host: | Goat |
| Applications: | Pep-ELISA/WB/IF/IHC |
| Reactivity: | Human/Mouse/Dog/Pig |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-GAPDH Loading Control (C-Term) is suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:4000. WB: Approx 35kDa band observed in lysates of cell line HEK293 and LNCaP, and approx. 37kDa in lysates of cell lines A549 and HeLa, and in Human Liver lysates (calculated MW of 36.1kDa accor |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | GAPDH |
| Gene ID: | 2597 |
| Uniprot ID: | G3P_HUMAN |
| Immunogen Region: | C-Term |
| Accession Number: | NP_002037.2 |
| Specificity: | GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting. |
| Immunogen Sequence: | HQVVSSDFNSDT |
| Function | Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. |
| Protein Name | Glyceraldehyde-3-Phosphate Dehydrogenase Gapdh Peptidyl-Cysteine S-Nitrosylase Gapdh |
| Database Links | Reactome: R-HSA-70171 Reactome: R-HSA-70263 |
| Cellular Localisation | Cytoplasm Cytosol Nucleus Perinuclear Region Membrane Cytoskeleton Translocates To The Nucleus Following S-Nitrosylation And Interaction With Siah1 Which Contains A Nuclear Localization Signal Postnuclear And Perinuclear Regions |
| Alternative Antibody Names | Anti-Glyceraldehyde-3-Phosphate Dehydrogenase antibody Anti-Gapdh antibody Anti-Peptidyl-Cysteine S-Nitrosylase Gapdh antibody Anti-GAPDH antibody Anti-GAPD antibody Anti-CDABP0047 antibody Anti-OK antibody Anti-SW-cl.12 antibody |
Information sourced from Uniprot.org