• Immunofluorescence analysis of paraffin-embedded mouse liver using F10 Rabbit polyclonal antibody (STJ117251) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of paraffin-embedded rat liver using F10 Rabbit polyclonal antibody (STJ117251) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using F10 Rabbit polyclonal antibody (STJ117251) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-F10 antibody (41-300) (STJ117251)

SKU:
STJ117251

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-F10 (41-300) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: F10
Gene ID: 2159
Uniprot ID: FA10_HUMAN
Immunogen Region: 41-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 41-300 of human F10 (NP_000495.1).
Immunogen Sequence: ANSFLEEMKKGHLERECMEE TCSYEEAREVFEDSDKTNEF WNKYKDGDQCETSPCQNQGK CKDGLGEYTCTCLEGFEGKN CELFTRKLCSLDNGDCDQFC HEEQNSVVCSCARGYTLADN GKACIPTGPYPCGKQTLERR KRSVAQATSSSGEAPDSITW KPYDAADLDPTENPFDLLDF NQTQPERGDNNLTRIVGGQE CKDGECPWQALLINEENEGF CGGTILSEFYILTAAHCLY
Tissue Specificity Plasma.synthesized in the liver.
Post Translational Modifications The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium. N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. Proteolytically cleaved and activated by cathepsin CTSG. The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway). The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
Function Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.
Protein Name Coagulation Factor X
Stuart Factor
Stuart-Prower Factor Cleaved Into - Factor X Light Chain - Factor X Heavy Chain - Activated Factor Xa Heavy Chain
Database Links Reactome: R-HSA-140834
Reactome: R-HSA-140837
Reactome: R-HSA-140875
Reactome: R-HSA-159740
Reactome: R-HSA-159763
Reactome: R-HSA-159782
Reactome: R-HSA-9672383
Reactome: R-HSA-9672396
Reactome: R-HSA-9673202
Cellular Localisation Secreted
Alternative Antibody Names Anti-Coagulation Factor X antibody
Anti-Stuart Factor antibody
Anti-Stuart-Prower Factor Cleaved Into - Factor X Light Chain - Factor X Heavy Chain - Activated Factor Xa Heavy Chain antibody
Anti-F10 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance