• Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Estrogen Receptor-alpha Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of lysates from 293 cells, using Estrogen Receptor-alpha Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of 293 cells using ER Alpha Polyclonal Antibody diluted at 1：2000
  • Immunofluorescence analysis of A549 cells, using Estrogen Receptor-alpha Antibody. The picture on the right is blocked with the synthesized peptide.
  • Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1, ER Alpha Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Western blot analysis of various cells using ER Alpha Polyclonal Antibody diluted at 1：2000
  • Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, ER Alpha Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1, ER Alpha Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunofluorescence analysis of rat-spleen tissue. 1, ER Alpha Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of rat-lung tissue. 1, ER Alpha Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of Hela cell. 1, ER Alpha Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Caspase-8 monoclonal antibody (2G12) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-ESR1 antibody (501-550 aa) (STJ92999)

SKU:
STJ92999

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Estrogen receptor (501-550 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ESR1
Gene ID: 2099
Uniprot ID: ESR1_HUMAN
Immunogen Region: 501-550 aa
Specificity: ER Alpha Polyclonal Antibody detects endogenous levels of ER Alpha protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Estrogen Receptor-alpha at the amino acid range 501-550
Post Translational Modifications Phosphorylated by cyclin A/CDK2 and CK1. Phosphorylation probably enhances transcriptional activity. Self-association induces phosphorylation. Dephosphorylation at Ser-118 by PPP5C inhibits its transactivation activity. Phosphorylated by LMTK3 in vitro. Glycosylated.contains N-acetylglucosamine, probably O-linked. Ubiquitinated.regulated by LATS1 via DCAF1 it leads to ESR1 proteasomal degradation. Deubiquitinated by OTUB1. Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization. Demethylated by JMJD6 at Arg-260. Palmitoylated (isoform 3). Not biotinylated (isoform 3). Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation, but not for signaling mediated by the nuclear hormone receptor.
Function Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements.the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3. Isoform 3: Involved in activation of NOS3 and endothelial nitric oxide production. Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full-length receptor. Binds to ERE and inhibits isoform 1.
Protein Name Estrogen Receptor
Er
Er-Alpha
Estradiol Receptor
Nuclear Receptor Subfamily 3 Group A Member 1
Database Links Reactome: R-HSA-1251985
Reactome: R-HSA-1257604
Reactome: R-HSA-2219530
Reactome: R-HSA-383280
Reactome: R-HSA-4090294
Reactome: R-HSA-5689896
Reactome: R-HSA-6811558
Reactome: R-HSA-8866910
Reactome: R-HSA-8931987
Reactome: R-HSA-8939211
Reactome: R-HSA-8939256
Reactome: R-HSA-8939902
Reactome: R-HSA-9009391
Reactome: R-HSA-9018519
Cellular Localisation Isoform 1: Nucleus
Cytoplasm
Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
A Minor Fraction Is Associated With The Inner Membrane
Isoform 3: Nucleus
Single-Pass Type I Membrane Protein
Associated With The Inner Membrane Via Palmitoylation (Probable)
At Least A Subset Exists As A Transmembrane Protein With A N-Terminal Extracellular Domain
Nucleus
Golgi Apparatus
Colocalizes With Zdhhc7 And Zdhhc21 In The Golgi Apparatus Where Most Probably Palmitoylation Occurs
Associated With The Plasma Membrane When Palmitoylated
Alternative Antibody Names Anti-Estrogen Receptor antibody
Anti-Er antibody
Anti-Er-Alpha antibody
Anti-Estradiol Receptor antibody
Anti-Nuclear Receptor Subfamily 3 Group A Member 1 antibody
Anti-ESR1 antibody
Anti-ESR antibody
Anti-NR3A1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance