| Host: | Goat |
| Applications: | Pep-ELISA/WB/IHC |
| Reactivity: | Human/Mouse/Rat/Dog/Pig/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-ENC1 (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 70kDa band observed in Rat Ovary lysates (calculated MW of 66.1kDa according to Rat NP_001003401.1. Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour. Prelimin |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | ENC1 |
| Gene ID: | 8507 |
| Uniprot ID: | ENC1_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_003624.1; NP_001243505.1 |
| Specificity: | This antibody is expected to recognize both reported isoforms (NP_003624.1; NP_001243505.1). Reported variants represent identical protein: NP_001243503.1, NP_001243504.1, NP_003624.1. |
| Immunogen Sequence: | TSVSHDKLPKVQC |
| Post Translational Modifications | Ubiquitinated by E3 ubiquitin ligase complex formed by CUL3 and RBX1 and probably targeted for proteasome-independent degradation. Quinone-induced oxidative stress increases its ubiquitination. |
| Function | Actin-binding protein involved in the regulation of neuronal process formation and in differentiation of neural crest cells. Down-regulates transcription factor NF2L2/NRF2 by decreasing the rate of protein synthesis and not via a ubiquitin-mediated proteasomal degradation mechanism. |
| Protein Name | Ectoderm-Neural Cortex Protein 1Enc-1Kelch-Like Protein 37Nuclear Matrix Protein Nrp/BP53-Induced Gene 10 Protein |
| Cellular Localisation | Nucleus MatrixCytoplasmCytoskeleton |
| Alternative Antibody Names | Anti-Ectoderm-Neural Cortex Protein 1 antibodyAnti-Enc-1 antibodyAnti-Kelch-Like Protein 37 antibodyAnti-Nuclear Matrix Protein Nrp/B antibodyAnti-P53-Induced Gene 10 Protein antibodyAnti-ENC1 antibodyAnti-KLHL37 antibodyAnti-NRPB antibodyAnti-PIG10 antibody |
Information sourced from Uniprot.org