| Host: | Rabbit |
| Applications: | ELISA |
| Reactivity: | Human/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | Eif2ak2 |
| Gene ID: | 19106 |
| Uniprot ID: | E2AK2_MOUSE |
| Specificity: | Recombinant protein of mouse Eif2ak2 |
| Tissue Specificity | Expressed in heart, lung, brain, kidney, testes, thymus and bone marrow. |
| Post Translational Modifications | Autophosphorylated on several Ser, Thr and Tyr residues. Autophosphorylation of Thr-414 is dependent on Thr-409 and is stimulated by dsRNA binding and dimerization. Autophosphorylation apparently leads to the activation of the kinase. Tyrosine autophosphorylation is essential for efficient dsRNA-binding, dimerization, and kinase activation. |
| Function | IFN-induced dsRNA-dependent serine/threonine-protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) and plays a key role in the innate immune response to viral infection. Inhibits viral replication via the integrated stress response (ISR): EIF2S1/eIF-2-alpha phosphorylation in response to viral infection converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, resulting to a shutdown of cellular and viral protein synthesis, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activator ATF4. Exerts its antiviral activity on a wide range of DNA and RNA viruses including west nile virus (WNV), sindbis virus (SV), foot-and-mouth virus (FMDV), semliki Forest virus (SFV) and lymphocytic choriomeningitis virus (LCMV). Also involved in the regulation of signal transduction, apoptosis, cell proliferation and differentiation: phosphorylates other substrates including p53/TP53, PPP2R5A, DHX9, ILF3, and IRS1. In addition to serine/threonine-protein kinase activity, also has tyrosine-protein kinase activity and phosphorylates CDK1 at 'Tyr-4' upon DNA damage, facilitating its ubiquitination and proteasomal degradation. Either as an adapter protein and/or via its kinase activity, can regulate various signaling pathways (p38 MAP kinase, NF-kappa-B and insulin signaling pathways) and transcription factors (JUN, STAT1, STAT3, IRF1, ATF3) involved in the expression of genes encoding pro-inflammatory cytokines and IFNs. Activates the NF-kappa-B pathway via interaction with IKBKB and TRAF family of proteins and activates the p38 MAP kinase pathway via interaction with MAP2K6. Can act as both a positive and negative regulator of the insulin signaling pathway (ISP). Negatively regulates ISP by inducing the inhibitory phosphorylation of insulin receptor substrate 1 (IRS1) at 'Ser-312' and positively regulates ISP via phosphorylation of PPP2R5A which activates FOXO1, which in turn up-regulates the expression of insulin receptor substrate 2 (IRS2). Can regulate NLRP3 inflammasome assembly and the activation of NLRP3, NLRP1, AIM2 and NLRC4 inflammasomes. Plays a role in the regulation of the cytoskeleton by binding to gelsolin (GSN), sequestering the protein in an inactive conformation away from actin. |
| Protein Name | Interferon-Induced - Double-Stranded Rna-Activated Protein Kinase Eukaryotic Translation Initiation Factor 2-Alpha Kinase 2 Eif-2a Protein Kinase 2 Interferon-Inducible Rna-Dependent Protein Kinase P1/Eif-2a Protein Kinase Protein Kinase Rna-Activated Pkr Protein Kinase R Serine/Threonine-Protein Kinase Tik Tyrosine-Protein Kinase Eif2ak2 P68 Kinase |
| Database Links | Reactome: R-MMU-1169408 Reactome: -MMU-9833482 |
| Cellular Localisation | Cytoplasm Nucleus Perinuclear Region |
| Alternative Antibody Names | Anti-Interferon-Induced - Double-Stranded Rna-Activated Protein Kinase antibody Anti-Eukaryotic Translation Initiation Factor 2-Alpha Kinase 2 antibody Anti-Eif-2a Protein Kinase 2 antibody Anti-Interferon-Inducible Rna-Dependent Protein Kinase antibody Anti-P1/Eif-2a Protein Kinase antibody Anti-Protein Kinase Rna-Activated antibody Anti-Pkr antibody Anti-Protein Kinase R antibody Anti-Serine/Threonine-Protein Kinase Tik antibody Anti-Tyrosine-Protein Kinase Eif2ak2 antibody Anti-P68 Kinase antibody Anti-Eif2ak2 antibody Anti-Pkr antibody Anti-Prkr antibody Anti-Tik antibody |
Information sourced from Uniprot.org