Western blot analysis of lysates from wild type (WT) and 293T cells transfected with E-Selectin/CD62E using E-Selectin/CD62E Rabbit polyclonal antibody (STJ11105535) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-E-Selectin/CD62E antibody (22-557) (STJ11105535)

SKU:
STJ11105535
£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:5000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Sele
Gene ID: 20339
Uniprot ID: LYAM2_MOUSE
Immunogen Region: 22-557
Specificity: Recombinant Protein corresponding to a sequence within amino acids 22-557 of mouse E-Selectin/CD62E (NP_035475.2).
Immunogen Sequence: WYYNASSELMTYDEASAYCQ RDYTHLVAIQNKEEINYLNS NLKHSPSYYWIGIRKVNNVW IWVGTGKPLTEEAQNWAPGE PNNKQRNEDCVEIYIQRTKD SGMWNDERCNKKKLALCYTA SCTNASCSGHGECIETINSY TCKCHPGFLGPNCEQAVTCK PQEHPDYGSLNCSHPFGPFS YNSSCSFGCKRGYLPSSMET TVRCTSSGEWSAPAPACHVV ECEALTHPAHGIRKCSSNP
Function Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.
Protein Name E-Selectin
Cd62 Antigen-Like Family Member E
Endothelial Leukocyte Adhesion Molecule 1
Elam-1
Leukocyte-Endothelial Cell Adhesion Molecule 2
Lecam2
Cd Antigen Cd62e
Database Links Reactome: R-MMU-202733
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-E-Selectin antibody
Anti-Cd62 Antigen-Like Family Member E antibody
Anti-Endothelial Leukocyte Adhesion Molecule 1 antibody
Anti-Elam-1 antibody
Anti-Leukocyte-Endothelial Cell Adhesion Molecule 2 antibody
Anti-Lecam2 antibody
Anti-Cd Antigen Cd62e antibody
Anti-Sele antibody
Anti-Elam-1 antibody

Information sourced from Uniprot.org

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