• Western blot analysis of Cytokeratin-7. Sample: Protein treated by RIPA Lysis Buffer. Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:3000, RT, 1h.
  • Immunohistochemistry analysis of Cytokeratin-7. Sample: Human stomach, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:500, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT 1h.
  • Immunohistochemistry analysis of Cytokeratin-7. Sample: mouse stomach, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:500, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT 1h.
  • Immunohistochemistry analysis of Cytokeratin-7. Sample: Rat stomach, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:500, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT 1h.
  • IF analysis of Cytokeratin-7. Sample: mouse uterus, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:100, 4C overnight. Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG, 1:200 RT 1h.
  • IF analysis of Cytokeratin-7. Sample: mouse uterus, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:100, 4C overnight. Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG, 1:200 RT 1h.
  • IF analysis of Cytokeratin-7. Sample: Rat stomach, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:100, 4C overnight. Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG, 1:200 RT 1h.

Anti-Cytokeratin 7 antibody [SM0465] (STJA0010465)

SKU:
STJA0010465

£170.50 - £233.50
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Host: Mouse
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Cytokeratin 7 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: SM0465
Conjugation: Unconjugated
Isotype: IgG2ak
Formulation: PBS with 0.02% sodium azide, 100 Mu g/ml BSA and 50% glycerol.
Purification: Affinity purification
Dilution Range: WB (H, M, R) 1:500-1:1000
IHC/IF (H, M, R) 1:500-1:1000/1:100-1:500
Storage Instruction: Store at-20C for up to one year, and avoid repeated freeze-thaw cycles.
Gene Symbol: Krt7
Gene ID: 110310
Uniprot ID: K2C7_MOUSE
Immunogen: Recombinant protein corresponding to Mouse Cytokeratin 7
Post Translational Modifications Arg-15 is dimethylated, probably to asymmetric dimethylarginine.
Function Blocks interferon-dependent interphase and stimulates DNA synthesis in cells.
Protein Name Keratin - Type Ii Cytoskeletal 7
Cytokeratin-7
Ck-7
Keratin-7
K7
Type-Ii Keratin Kb7
Database Links Reactome: R-MMU-6805567
Reactome: -MMU-6809371
Alternative Antibody Names Anti-Keratin - Type Ii Cytoskeletal 7 antibody
Anti-Cytokeratin-7 antibody
Anti-Ck-7 antibody
Anti-Keratin-7 antibody
Anti-K7 antibody
Anti-Type-Ii Keratin Kb7 antibody
Anti-Krt7 antibody
Anti-Krt2-7 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance