• Western blot analysis of lysates from LOVO cells, using Cytochrome P450 7B1 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemical analysis of paraffin-embedded Human skeletal muscle. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-CYP7B1 antibody (101-150 aa) (STJ92606)

SKU:
STJ92606

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Cytochrome P450 7B1 (101-150 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CYP7B1
Gene ID: 9420
Uniprot ID: CP7B1_HUMAN
Immunogen Region: 101-150 aa
Specificity: CYP7B1 Polyclonal Antibody detects endogenous levels of CYP7B1 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Cytochrome P450 7B1 at the amino acid range 101-150
Function A cytochrome P450 monooxygenase involved in the metabolism of endogenous oxysterols and steroid hormones, including neurosteroids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR.NADPH-ferrihemoprotein reductase). Catalyzes the hydroxylation of carbon hydrogen bonds of steroids with a preference for 7-alpha position. Usually metabolizes steroids carrying a hydroxy group at position 3, functioning as a 3-hydroxy steroid 7-alpha hydroxylase. Hydroxylates oxysterols, including 25-hydroxycholesterol and (25R)-cholest-5-ene-3beta,26-diol toward 7-alpha hydroxy derivatives, which may be transported to the liver and converted to bile acids. Via its product 7-alpha,25-dihydroxycholesterol, a ligand for the chemotactic G protein-coupled receptor GPR183/EBI2, regulates B cell migration in germinal centers of lymphoid organs, thus guiding efficient maturation of plasma B cells and overall antigen-specific humoral immune response. 7-alpha hydroxylates neurosteroids, including 3beta-hydroxyandrost-5-en-17-one (dehydroepiandrosterone) and pregnenolone, both involved in hippocampus-associated memory and learning. Metabolizes androstanoids toward 6- or 7-alpha hydroxy derivatives.
Protein Name Cytochrome P450 7b1
24-Hydroxycholesterol 7-Alpha-Hydroxylase
25/26-Hydroxycholesterol 7-Alpha-Hydroxylase
3-Hydroxysteroid 7-Alpha Hydroxylase
Oxysterol 7-Alpha-Hydroxylase
Database Links Reactome: R-HSA-192105
Reactome: R-HSA-193368
Reactome: R-HSA-193807
Reactome: R-HSA-211976
Reactome: R-HSA-5579013
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Microsome Membrane
Alternative Antibody Names Anti-Cytochrome P450 7b1 antibody
Anti-24-Hydroxycholesterol 7-Alpha-Hydroxylase antibody
Anti-25/26-Hydroxycholesterol 7-Alpha-Hydroxylase antibody
Anti-3-Hydroxysteroid 7-Alpha Hydroxylase antibody
Anti-Oxysterol 7-Alpha-Hydroxylase antibody
Anti-CYP7B1 antibody

Information sourced from Uniprot.org

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