• Western blot analysis of extracts of various cell lines, using CYP2A6 Antibody (STJ28378) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CYP2A6 antibody (215-494) (STJ28378)

SKU:
STJ28378

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CYP2A6 (215-494) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CYP2A6
Gene ID: 1548
Uniprot ID: CP2A6_HUMAN
Immunogen Region: 215-494
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 215-494 of human CYP2A6 (NP_000753.3).
Immunogen Sequence: STGQLYEMFSSVMKHLPGPQ QQAFQLLQGLEDFIAKKVEH NQRTLDPNSPRDFIDSFLIR MQEEEKNPNTEFYLKNLVMT TLNLFIGGTETVSTTLRYGF LLLMKHPEVEAKVHEEIDRV IGKNRQPKFEDRAKMPYMEA VIHEIQRFGDVIPMSLARRV KKDTKFRDFFLPKGTEVYPM LGSVLRDPSFFSNPQDFNPQ HFLNEKGQFKKSDAFVPFSI GKRNCFGEGLARMELFLFF
Tissue Specificity Liver.
Function Exhibits a high coumarin 7-hydroxylase activity. Can act in the hydroxylation of the anti-cancer drugs cyclophosphamide and ifosphamide. Competent in the metabolic activation of aflatoxin B1. Constitutes the major nicotine C-oxidase. Acts as a 1,4-cineole 2-exo-monooxygenase. Possesses low phenacetin O-deethylation activity.
Protein Name Cytochrome P450 2a6
1 -4-Cineole 2-Exo-Monooxygenase
Cypiia6
Coumarin 7-Hydroxylase
Cytochrome P450 Iia3
Cytochrome P450(I
Database Links Reactome: R-HSA-211981
Reactome: R-HSA-211999
Cellular Localisation Endoplasmic Reticulum Membrane
Peripheral Membrane Protein
Microsome Membrane
Alternative Antibody Names Anti-Cytochrome P450 2a6 antibody
Anti-1 -4-Cineole 2-Exo-Monooxygenase antibody
Anti-Cypiia6 antibody
Anti-Coumarin 7-Hydroxylase antibody
Anti-Cytochrome P450 Iia3 antibody
Anti-Cytochrome P450(I antibody
Anti-CYP2A6 antibody
Anti-CYP2A3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance