• Western blot analysis of lysates from HeLa and 293 cells, using CKI-gamma2 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of HeLa cells using Casein Kinase I Gamma 2 Polyclonal Antibody

Anti-CSNK1G2-Gamma antibody (10-90 aa, N-terminal) (STJ92014)

SKU:
STJ92014

Current Stock:
Host: Rabbit
Applications: WB, IHC-P, IF, ELISA
Reactivity: Human, Mouse, Rat
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit polyclonal antibody Anti-Casein kinase I isoform gamma-2 (10-90 aa, N-terminal) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Concentration: 1 mg/ml
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Gene Symbol: CSNK1G2
Gene ID: 1455
Uniprot ID: KC1G2_HUMAN
Immunogen Region: 10-90 aa, N-terminal
Specificity: Casein Kinase I gamma 2 polyclonal antibody detects endogenous levels of Casein Kinase I gamma 2 protein.
Immunogen: Synthesized peptide derived from human Casein Kinase Igamma2
Post Translational Modifications Autophosphorylated. Phosphorylated by aPKC which promotes dissociation from the cell cortex.
Function Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates COL4A3BP/CERT, MTA1 and SMAD3. Involved in brain development and vesicular trafficking and neurotransmitter releasing from small synaptic vesicles. Regulates fast synaptic transmission mediated by glutamate. SMAD3 phosphorylation promotes its ligand-dependent ubiquitination and subsequent proteasome degradation, thus inhibiting SMAD3-mediated TGF-beta responses. Hyperphosphorylation of the serine-repeat motif of COL4A3BP/CERT leads to its inactivation by dissociation from the Golgi complex, thus down-regulating ER-to-Golgi transport of ceramide and sphingomyelin synthesis. Triggers PER1 proteasomal degradation probably through phosphorylation.
Protein Name Casein Kinase I Isoform Gamma-2
Cki-Gamma 2
Database Links Reactome: R-HSA-1660661
Reactome: R-HSA-4641262
Cellular Localisation Cytoplasm
Cell Cortex
Alternative Antibody Names Anti-Casein Kinase I Isoform Gamma-2 antibody
Anti-Cki-Gamma 2 antibody
Anti-CSNK1G2 antibody
Anti-CK1G2 antibody

Information sourced from Uniprot.org

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