• Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using Collagen IV alpha5 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of the lysates from COLO205 cells using 5-HT-6 antibody.
  • Immunofluorescence analysis of HeLa cells, using Collagen IV alpha5 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-COL4A5 antibody (STJ92393)

Current Stock:
Host: Rabbit
Applications: IHC-P, IF, WB, ELISA
Reactivity: Human, Mouse
Short Description: Rabbit polyclonal primary antibody against COL4A5.
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG
Dilution Range: WB 1:500-2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Specificity: COL4A5 polyclonal antibody detects endogenous levels of COL4A5 protein.
Uniprot ID: CO4A5_HUMAN
Gene ID: 1287
Gene Symbol: COL4A5
Immunogen: Synthesized peptide derived from human COL4A5
Immunogen Region: 40-120 aa, N-terminal
Tissue Specificity Isoform 2 is found in kidney.
Post Translational Modifications Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens. The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues.
Function Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
Database Links Reactome: R-HSA-1442490
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Basement Membrane
Protein Name Collagen Alpha-5(Iv Chain
Alternative Names Anti-Collagen Alpha-5(Iv Chain antibody
Anti-COL4A5 antibody

Information sourced from Uniprot.org

6 months standard