Host: | Rabbit |
Applications: | WB/DB/ELISA |
Reactivity: | Human/Mouse/Rat |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Clonality: | Monoclonal |
Clone ID: | SRM |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3. |
Purification: | Affinity purification |
Concentration: | Lot specific |
Dilution Range: | WB:1:500-1:1000 DB:1:500-1:1000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
Gene Symbol: | COL4A4 |
Gene ID: | 1286 |
Uniprot ID: | CO4A4_HUMAN |
Immunogen Region: | 1573-1690 aa |
Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1573-1690 of human COL4A4/COL4A6/COL4A2/COL4A1 (NP_000083.3). |
Immunogen Sequence: | AQAVAVHSQDQSIPPCPQTW RSLWIGYSFLMHTGAGDQGG GQALMSPGSCLEDFRAAPFL ECQGRQGTCHFFANKYSFWL TTVKADLQFSSAPAPDTLKE SQAQRQKISRCQVCVKYS |
Tissue Specificity | Expressed in Bruch's membrane, outer plexiform layer, inner nuclear layer, inner plexiform layer, ganglion cell layer, inner limiting membrane and around the blood vessels of the retina (at protein level). Alpha 3 and alpha 4 type IV collagens are colocalized and present in kidney, eye, basement membranes of lens capsule, cochlea, lung, skeletal muscle, aorta, synaptic fibers, fetal kidney and fetal lung. PubMed:8083201 reports similar levels of expression of alpha 3 and alpha 4 type IV collagens in kidney, but PubMed:7523402 reports that in kidney levels of alpha 3 type IV collagen are significantly lower than those of alpha 4 type IV collagen. Highest levels of expression of alpha 4 type IV collagen are detected in kidney, calvaria, neuroretina and cardiac muscle. Lower levels of expression are observed in brain, lung and thymus, and no expression is detected in choroid plexus, liver, adrenal, pancreas, ileum or skin. |
Post Translational Modifications | Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens. The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues. |
Function | Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. |
Protein Name | Collagen Alpha-4(Iv Chain |
Database Links | Reactome: R-HSA-1442490 Reactome: R-HSA-1474244 Reactome: R-HSA-1650814 Reactome: R-HSA-186797 Reactome: R-HSA-2022090 Reactome: R-HSA-216083 Reactome: R-HSA-2214320 Reactome: R-HSA-2243919 Reactome: R-HSA-3000157 Reactome: R-HSA-3000171 Reactome: R-HSA-3000178 Reactome: R-HSA-419037 Reactome: R-HSA-8948216 |
Cellular Localisation | Secreted Extracellular Space Extracellular Matrix Basement Membrane Colocalizes With Col4a4 And Col4a5 In Gbm Tubular Basement Membrane (Tbm) And Synaptic Basal Lamina (Bl) |
Alternative Antibody Names | Anti-Collagen Alpha-4(Iv Chain antibody Anti-COL4A4 antibody |
Information sourced from Uniprot.org