Western blot analysis of lysates from MCF7 cells, using COA3 Rabbit polyclonal antibody (STJ116795) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-COA3 antibody (1-106) (STJ116795)

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STJ116795
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: COA3
Gene ID: 28958
Uniprot ID: COA3_HUMAN
Immunogen Region: 1-106
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-106 of human COA3 (NP_001035521.1).
Immunogen Sequence: MASSGAGDPLDSKRGEAPFA QRIDPTREKLTPEQLHSMRQ AELAQWQKVLPRRRTRNIVT GLGIGALVLAIYGYTFYSIS QERFLDELEDEAKAARARAL ARASGS
Function Core component of the MITRAC (mitochondrial translation regulation assembly intermediate of cytochrome c oxidase complex) complex, that regulates cytochrome c oxidase assembly. MITRAC complexes regulate both translation of mitochondrial encoded components and assembly of nuclear-encoded components imported in mitochondrion. Required for efficient translation of MT-CO1 and mitochondrial respiratory chain complex IV assembly.
Protein Name Cytochrome C Oxidase Assembly Factor 3 Homolog - Mitochondrial
Coiled-Coil Domain-Containing Protein 56
Mitochondrial Translation Regulation Assembly Intermediate Of Cytochrome C Oxidase Protein Of 12 Kda
Database Links Reactome: R-HSA-9864848
Cellular Localisation Mitochondrion Inner Membrane
Single-Pass Membrane Protein
Alternative Antibody Names Anti-Cytochrome C Oxidase Assembly Factor 3 Homolog - Mitochondrial antibody
Anti-Coiled-Coil Domain-Containing Protein 56 antibody
Anti-Mitochondrial Translation Regulation Assembly Intermediate Of Cytochrome C Oxidase Protein Of 12 Kda antibody
Anti-COA3 antibody
Anti-CCDC56 antibody
Anti-MITRAC12 antibody
Anti-HSPC009 antibody

Information sourced from Uniprot.org

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