![Anti-Clostridium difficile Toxin A antibody [EBS-I-100] (STJ16101048)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/149764/302508/STJ16101048_1__04490.1646940676.jpg?c=1 "Anti-Clostridium difficile Toxin A antibody [EBS-I-100] (STJ16101048)")
![Anti-Clostridium difficile Toxin A antibody [EBS-I-100] (STJ16101048)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/149764/302508/STJ16101048_1__04490.1646940676.jpg?c=1 "Anti-Clostridium difficile Toxin A antibody [EBS-I-100] (STJ16101048)")
| Host: | Mouse |
| Applications: | ELISA/IF/IHC |
| Reactivity: | C.Difficile |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-Clostridium difficile Toxin A is suitable for use in ELISA, Immunofluorescence and Immunohistochemistry research applications. |
| Clonality : | Monoclonal |
| Clone ID : | EBS-I-100 |
| Conjugation: | Unconjugated |
| Isotype: | IgG3k |
| Formulation: | PBS with 0.02% Sodium Azide. |
| Purification: | Affinity purified from tissue culture. |
| Concentration: | Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
| Dilution Range: | ELISA (solid phase: not known; tracer: 0, 001-100 µg/ml for 30 min at RT). Immunofluorescence (0.5-1 µg/ml). Immunohistology (1-2 µg/ml for 30 min at RT; an appropriate antigen retrieval method for staining of formalin-fixed tissues has not been esta |
| Storage Instruction: | Store for up to 1 year at 2-8°C upon receipt. |
| Immunogen: | A BALB/c mouse was immunized with C. difficile toxin A. Fusion partner: Sp2/0. |
| Background | EBS-I-100 reacts with C. difficile Toxin A, but not with V. cholerae subunit a, V. cholerae toxin, Pseudomonas aeruginosa exotoxin A, H-LT, P-LT. C. difficile is a major nosocomial pathogen that causes antibiotic-associated colitis and mediates inflammatory diarrhea by releasing two large protein enterotoxins (toxin A and toxin B) that are able to disrupt intestinal epithelial cells via their transferase activity and ability to monoglucosylate members of the Rho family. C. difficile toxin A is a toxin that is composed of 39 repeats that are responsible for binding to intestinal epithelial cell surface carbohydrates. C. difficile toxin A causes significant apoptosis of colonocytes which contributes to the formation of ulcers and pseudo-membranes in a pathway that involves p38-dependent activation of p53 and induction of p21, leading to cytochrome c release and caspase-3 activation through Bak activation. |
Information sourced from Uniprot.org