| Host: | Rabbit |
| Applications: | WB/IF/ICC/IP/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CKAP5 |
| Gene ID: | 9793 |
| Uniprot ID: | CKAP5_HUMAN |
| Immunogen Region: | 1763-2032 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1763-2032 of human CKAP5 (NP_001008938.1). |
| Immunogen Sequence: | LLHTLCKLKGPKILDHLTMI DNKNESELEAHLCRMMKHSM DQTGSKSDKETEKGASRIDE KSSKAKVNDFLAEIFKKIGS KENTKEGLAELYEYKKKYSD ADIEPFLKNSSQFFQSYVER GLRVIEMEREGKGRISTSTG ISPQMEVTCVPTPTSTVSSI GNTNGEEVGPSVYLERLKIL RQRCGLDNTKQDDRPPLTSL LSKPAVPTVASSTDMLHSKL SQLRESREQHQHSDLDSNQ |
| Tissue Specificity | Overexpressed in hepatomas and colonic tumors. Also expressed in skeletal muscle, brain, heart, placenta, lung, liver, kidney and pancreas. Expression is elevated in the brain.highly expressed in the Purkinje cell bodies of the cerebellum. |
| Function | Binds to the plus end of microtubules and regulates microtubule dynamics and microtubule organization. Acts as a processive microtubule polymerase. Promotes cytoplasmic microtubule nucleation and elongation. Plays a major role in organizing spindle poles. In spindle formation protects kinetochore microtubules from depolymerization by KIF2C and has an essential role in centrosomal microtubule assembly independently of KIF2C activity. Contributes to centrosome integrity. Acts as a component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension. Enhances the strength of NDC80 complex-mediated kinetochore-tip microtubule attachments. |
| Protein Name | Cytoskeleton-Associated Protein 5Colonic And Hepatic Tumor Overexpressed Gene ProteinCh-Tog |
| Database Links | Reactome: R-HSA-141444Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-2565942Reactome: R-HSA-380259Reactome: R-HSA-380270Reactome: R-HSA-380284Reactome: R-HSA-380320Reactome: R-HSA-5620912Reactome: R-HSA-5663220Reactome: R-HSA-68877Reactome: R-HSA-8854518Reactome: R-HSA-9648025 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeSpindle PoleSpindleChromosomeCentromereKinetochoreDetected On Centrosomes And Kinetochores During Interphase And Mitosis Independently From Tacc3 And ClathrinLocated To Spindle Poles And Microtubules During MitosisIn Complex With Tacc3 Localized To Microtubule Plus-Ends In Mitosis And InterphaseIn Complex With Tacc3 And Clathrin Localized To Inter-Microtubule Bridges In Mitotic SpindlesAccumulation Sites At Microtubule Plus Ends Protruded Approximately 100 Nm From Mapre1/Eb1 Sites In Interphase Cells |
| Alternative Antibody Names | Anti-Cytoskeleton-Associated Protein 5 antibodyAnti-Colonic And Hepatic Tumor Overexpressed Gene Protein antibodyAnti-Ch-Tog antibodyAnti-CKAP5 antibodyAnti-KIAA0097 antibody |
Information sourced from Uniprot.org