| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-CDT1/Dup (C-Term) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 60kDa band observed in Human Ovary lysates (calculated MW of 60.4kDa according to NP_112190.2). Recommended concentration: 1-3µg/ml. |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | CDT1 |
| Gene ID: | 81620 |
| Uniprot ID: | CDT1_HUMAN |
| Immunogen Region: | C-Term |
| Accession Number: | NP_112190.2 |
| Immunogen Sequence: | ARLAHQTRAEEGL |
| Function | Required for both DNA replication and mitosis. DNA replication licensing factor, required for pre-replication complex assembly. Cooperates with CDC6 and the origin recognition complex (ORC) during G1 phase of the cell cycle to promote the loading of the mini-chromosome maintenance (MCM) complex onto DNA to generate pre-replication complexes (pre-RC). Required also for mitosis by promoting stable kinetochore-microtubule attachments. Potential oncogene. |
| Protein Name | Dna Replication Factor Cdt1Double Parked HomologDup |
| Database Links | Reactome: R-HSA-68867Reactome: R-HSA-68949Reactome: R-HSA-68962Reactome: R-HSA-69052Reactome: R-HSA-69205 |
| Cellular Localisation | NucleusChromosomeCentromereKinetochoreTransiently Localizes To Kinetochores During Prometaphase And Metaphase |
| Alternative Antibody Names | Anti-Dna Replication Factor Cdt1 antibodyAnti-Double Parked Homolog antibodyAnti-Dup antibodyAnti-CDT1 antibody |
Information sourced from Uniprot.org